雌激素受体α和Bcl-2在阿尔茨海默病患者海马中的共存  

Estrogen Receptor α and Colocalization of Bcl-2 in Hippocampus of Alzheimer′s Disease

作　　者：曾梅[1] 鲁亚平[1] 许浩[1] 周江宁[1] 

机构地区：[1]中国科技大学生命科学学院神经生物学系,合肥230027

出　　处：《中国医学科学院学报》2004年第2期108-111,i003,共5页Acta Academiae Medicinae Sinicae

基　　金：国家重点基础性研究项目(973项目)(G1999054007)资助~~

摘　　要：目的 探讨雌激素通过雌激素受体 (estrogen receptor ER )调节细胞凋亡的可能机制。方法 利用荧光免疫组织化学的方法,研究了 10例阿尔茨海默病 Alzheim er's disease AD 患者和 10例对照受试者海马中 Bcl-2的分布及 Bcl-2和 ER α的共存现象。结果 Bcl-2主要在 AD 患者和对照受试者海马 CA 3和 CA 4亚区的锥体层神经元中广泛表达,且多分布于胞质和突起,胞核较少。星型胶质细胞中也可检测到 Bcl-2免疫活性,AD 组中大量表达,而对照组中很少表达。免疫组织化学荧光双标显示,大部分被 Bcl-2免疫标记的神经元也表达 ER α。结论 在 AD 患者海马的神经元和星型胶质细胞中,雌激素可能作为细胞凋亡的一个调节子,通过 ER α来调节 Bcl-2表达,行使其神经保护作用。Objective To investigate the possible mechanism by which estrogen regulates apoptosis through the estrogen receptor. Methods By means of fluorescence immunocytochemistry, the present study investigated the distribution of Bcl-2 and the colocolization of Bcl-2 and ERα immunoreactivity in the hippocampus of 10 Alzheimer′s disease(AD)patients and 10 aged controls. Results Bcl-2 immunoreac-tivity was widely distributed in neurons, concentrating predominantly on the subfields CA3 and CA4 in the stratum pyramidale of hippocampus both in controls and in AD patients. Bcl-2 staining in the labeled neuron was observed mainly in the cytoplasm and neuritic processes, but a few nuclei were also positive. Bcl-2 labeling was also detected in the astrocytes mainly in AD, but sparsely in controls. Double-labeled fluorescence immunocytochemistry showed that most Bcl-2-immunolabeled neurons also exhibited positive staining for ERα. Conclusions Estrogen may function as a regulator of apoptosis to modulate the expression of Bcl-2 in neurons and astrocytes in hippocampus of AD through ERα.

关 键 词：Bcl-2雌激素 雌激素受体Α 阿尔茨海默病 细胞凋亡海马 

分 类 号：R749.16[医药卫生—神经病学与精神病学]