人胚鼻咽粘膜异种移植及二亚硝基哌嗪的诱癌研究  

INVESTIGATION OF HUMAN FETAL NASOPHARYNGEAL MUCOSAL XENOGRAFT AND DNP CARCINOGENESIS

作　　者：吴枫 沈淑静 

机构地区：广东湛江医学院肿瘤研究室 广东湛江医学院肿瘤研究室 湛江 524023 湛江 524023 湛江 524023

出　　处：《癌症》1992年第1期6-8,T000,共4页Chinese Journal of Cancer

基　　金：卫生部“七·五”攻关项目资助课题

摘　　要：人胚鼻咽粘膜移植于裸鼠皮下后可长期存活并保持其原有组织结构和功能,在此基础上用二亚硝基哌嗪皮下给药法诱发其癌变。在40周内,低剂量多次给药组(12例)见不典型增生6例次,原位癌1例,早期浸润癌1例;一次性给药组(11例)见不典型增生5例次,原位癌3例。用抗P_(21)单抗检测rasP_(21)蛋白表达,发现异型增生的基底细胞较正常基底细胞表达增强,可作为癌前病变的指标之一。The transplanted normal human fetal nasopharyngeal tissues survived and maintained their original structure and mucussecreting function for a long time in nude mice. Serial changes of transplants were found from 4 to 40 weeks after subcutaneous administration of N,N' -dinitrosopiperazine (DNP). The experiment showed 5 cases of dysplasia, 1 of early infiltrating carcinoma, 1 of carcinoma-in-situ in serial DNP-inject-ion group (12 animals), and 5 of dysplasia, 3 of carcinoma-in -situin DNP once injection group (11 animals). We found also that the ras P21 expression of transplants was higher in basal cells of dysplasia than normal ones. The results pointed out that the change could be one of the manifestations of precancerous lesions.

关 键 词：鼻肿瘤 移植 裸鼠 二亚硝基哌嗪 

分 类 号：R73－354[医药卫生—肿瘤]