幽门螺杆菌相关性胃疾病胃蛋白酶原C的表达研究  

Expression of pepsinogen C in Helicobacter pylori-associated gastric lesions

作　　者：宁佩芳[1] 刘惠杰[1] 袁媛[1] 

机构地区：[1]中国医科大学附属一院肿瘤研究所,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2004年第5期1089-1091,共3页World Chinese Journal of Digestology

基　　金：国家十五科技攻关资助项目;No.2001BA703B06(B)国家自然科学基金资助项目;No.30171054~~

摘　　要：目的:探讨幽门螺杆菌(Hpylori)相关性胃疾病胃蛋白酶原C 的表达及其意义. 方法:采用免疫组织化学染色法检测318例胃黏膜标本中胃蛋白酶原C的表达情况;采用HE染色、ELISA及PCR方法检测Hpylori感染情况. 结果:Hpylori阳性组浅表陛胃炎胃蛋白酶原C抗原的过表达率高于Hpylori阴性组(P<0.05,X2=0.032,28/33vs 15/25),Hpylori阳性组萎缩性胃炎胃蛋白酶原C的表达率低于Hpylori阴性组(P<0.01,X2=0.003 4/61 vs9/30), Hpylori阳性组的异型增生和胃癌胃蛋白酶原C的表达率与Hpylori阴性组相比有下降趋势(P>0.05). 结论:Hpylori感染与胃蛋白酶原C的表达密切相关,在胃黏膜炎症中胃蛋白酶原C的表达增加;在萎缩性胃炎、异型增生和胃癌中胃蛋白酶原C的表达降低.AIM: To investigate the expression of pepsinogen C and its relation with H pylori infection in gastric cancer and precancerous lesions. METHODS: The method of immunohistochemistry was used to examine the expression of pepsinogen C in 318 cases of stomach mucosa; the H pylori infection was determined by H-E stain, PCR and ELISA. RESULTS: The rate of PGC over-expression in group of superficial gastritis of H pylori infection was higher than that of non-infection (P<0.05, 28/33 vs 15/25). The positive rate of PGC in group of atrophic gastritis of H pylori infection was lower than that of non-infection (P<0.01, 4/61 vs 9/30) and so were in dysplasia and gastric cancer. CONCLUSION: There is a relationship between the H pylori infection and the expression of PGC in gastric mucosa. The expression of PGC increases in superficial gastritis and decreases in atrophic gastritis, dysplasia and gastric cancer with H pylori infection.

关 键 词：幽门螺杆菌 胃疾病 胃蛋白酶原C 基因表达 胃癌 

分 类 号：R573[医药卫生—消化系统]