一株嗜热子囊菌产生的碱性耐热过氧化氢酶及其应用潜力  

Thermo-alkali-stable Catalase from Thermoascus aurantiacus and Its Potential Use in Textile Bleaching Process

作　　者：方芳[1,2] 李寅[1,2] 堵国成[2] 张娟[1,2] 陈坚[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室 [2]江南大学生物工程学院环境生物技术研究室,无锡214036

出　　处：《生物工程学报》2004年第3期423-428,共6页Chinese Journal of Biotechnology

基　　金：国家高技术研究发展计划 ( 863 )项目资助 (No .2 0 0 3AA3 2 2 0 5 0 )~~

摘　　要：研究了一株嗜热子囊菌产过氧化氢酶的摇瓶发酵条件 ,并对其在纺织工业中的应用潜力进行了评价。以2 0g L糊精和 1% (V V)乙醇为混合碳源时 ,过氧化氢酶酶活达到 15 94u mL ,比以糊精和乙醇单独为碳源时过氧化氢酶的活力之和还高 2 3%。改变培养基的初始pH、提高发酵液中的溶氧水平及添加外源过氧化氢 ,过氧化氢酶的产量进一步提高到 2 76 2u mL ,比优化前提高了 5 8倍。将嗜热子囊菌的过氧化氢酶同来源于牛肝、黑曲霉的过氧化氢酶进行了热 (70℃ ,80℃ ,90℃ )、碱 (pH 9 0 ,pH 10 0 ,pH 11 0 )稳定性的比较。结果显示 ,产自嗜热子囊菌的过氧化氢酶对高温和强碱性的耐受性能明显优于其它来源的酶 ,在纺织染整工艺中具有良好的应用潜力。The effect of growth and fermentation conditions on the production of catalase by T. aurantiacus WSH 03-01 was investigated in shaking flasks. Catalase activity reached 1594 u/mL when the culture was grown on a complex carbon source containing 20 g/L dextrin and 1%(V/V) ethanol, which was 23% higher than the sum produced on 20 g/L dextrin and 1%(V/V) ethanol, respectively. It was concluded that dextrin might act as a major carbon source in the complex, while ethanol was rather a stimulator than a carbon source. The stimulation effect of ethanol on catalase production was postulated to be two aspects; catalase-dependent alcohol metabolism is activated by acute alcohol, thus more catalase need to be synthesized for that use, named direct induction. As for indirect induction, which may result from little amount of H 2O 2 generation in process of NADH regeneration in respiratory chain. Peptone was shown to be a favorable nitrogen source for catalase production and its optimum concentration was found to be 10 g/L. Catalase production by T. aurantiacus WSH 03-01 was further improved by optimizing the initial pH, volume of medium in flasks as well as the concentration of external H 2O 2. Under the optimum culture conditions, the activity of catalase reached 2762 u/mL, which was nearly 6.8 times higher than that of the initiate conditions. Furthermore, the potential application of this novel catalase in the treatment of textile bleaching effluents was evaluated. Thermo-and alkaline stability of this catalase was compared with the commercial available catalases produced from bovine and Aspergillus niger. The crude en^zyme from T. aurantiacus WSH 03-01 showed stronger stabilities at (70 ℃, 80 ℃, 90 ℃) and (pH 9.0, pH 10.0, pH 11.0) than the other two types of catalases, indicating a great application potential in the clean production process of textile industry.

关 键 词：过氧化氢酶 嗜热子囊菌 混合碳源 过氧化氢 热、碱稳定性 

分 类 号：Q946[生物学—植物学]