紫外分光光度法测定大豆总异黄酮的含量  

DETERMINATION OF SOYBEAN ISOFLAVONE WITH ULTRA-VIOLET SPECTROPHOTOMETRY

作　　者：袁金斌[1] 卢建中 

机构地区：[1]江西中医学院中药制药研究中心,南昌330006 [2]江中制药集团技术部,南昌330077

出　　处：《大豆科学》2004年第2期147-150,共4页Soybean Science

摘　　要：建立了测定大豆提取物及以大豆展品黄酮为原料的保健食品中大豆总异黄酮的紫外分光光度法。以染料木素为标准品 ,优选出 32 5nm为测量波长 ,以染料木素计量试样中的大豆总异黄酮。大豆提取物中大豆总异黄酮的含量为 38 1 % ,加标回收率为 1 0 2 3% ;某保健品中大豆总总异黄酮的含量为 4 76 % ,加标回收率为 99 8%。方法简便、准确、重现性好 。A UV spectrphotometry used for determination of soybean isoflavone was established. Genistein was selected as the reference material, and the samples were determined at 325 nm. Influencing factors were dicussed and optimized. The total content of soybean isoflavones in soybean extracts is 38.1% with mean recovery 102.3%, and the result for the function food is 4.76% with mean recovery 99.9%.This method is suitable for the routine analysis and quality control of the function food with easy-to-handle, good accuracy and reproducibility.

关 键 词：紫外分光光度法 大豆 异黄酮 保健食品 

分 类 号：O657.3[理学—分析化学]