通过噬菌体载体筛选胶质瘤细胞结合的内化短肽  

Selection of targeted glioblastoma tumor cell-binding and internalizing peptides through phage display vector

作　　者：王冰[1] 钟雪云[1] 秦艳芳[1] 钟瑛[1] 于莉娜[1] 

机构地区：[1]暨南大学医学院病理学教研室,广东广州510632

出　　处：《中国病理生理杂志》2004年第5期752-756,共5页Chinese Journal of Pathophysiology

基　　金：国家科技部重大基础研究前期研究专项(2 0 0 2ccc0 4 0 0 );广州市科技局科研重点立项课题 (2 0 0 1-E -0 1- 2 )

摘　　要：目的 :寻找与神经胶质细胞瘤细胞系SWO - 38特异性结合并内化的短肽序列。方法 :利用噬菌体随机 12肽库对肿瘤细胞进行 5轮全细胞筛选 ,并分析筛选后单克隆对肿瘤细胞的特异性结合能力。提取单克隆DNA ,测序 ,推导出短肽序列。结果 :5轮筛选后的噬菌体库及所挑选 13个单克隆中有 10个对胶质瘤细胞有特异性的结合 ,并测序得到两条重复性高的多肽序列。结论 :通过噬菌体随机肽库对肿瘤细胞进行全细胞筛选得到的噬菌体多肽能高特异性与肿瘤细胞结合 。AIM: To isolate peptides targeted binding and internalizing into glioblastoma cell line SWO-38. METHODS: Tumor cells were screened five rounds of whole cell screen through the Ph.D.-12 phage display library. The monoclone specific binding efficiency to the tumor cell was analyzed, and the DNA of phages were extracted, sequenced and translated to the sequences of amino acid. RESULTS: In the phage library after five rounds of screen , 10 of 13 monoclones had highly selective binding to SWO-38 cells. We found two repeated peptide sequences. CONCLUSION: Whole cell screening against tumor cells through random phage peptide library can obtain phage peptides with highly specific binding and internalizing ability. The peptides could be used as a therapy vector for tumor targeted delivery.

关 键 词：胶质母细胞瘤 肽库 

分 类 号：R363[医药卫生—病理学]