MAGE-1、MAGE-3基因在胃癌和胃活检组织中的表达及意义  

Expression of MAGE-1 and MAGE-3 genes in gastric cancer and gastric biopsy tissues and its clinical significance

作　　者：陈雪华[1] 刘炳亚[1] 张冬青[2] 张奕[1] 张轶[1] 李建芳[1] 朱正纲[1] 

机构地区：[1]上海第二医科大学附属瑞金医院外科上海消化外科研究所,上海200025 [2]上海市免疫学研究所,上海200025

出　　处：《细胞与分子免疫学杂志》2004年第3期310-313,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目 (No .30 1 70 91 5)

摘　　要：目的 :检测黑色素瘤抗原基因 1、 3(MAGE 1和MAGE 3)在胃癌中的表达 ,探索其在胃癌免疫治疗中的应用价值。方法 :采用RT PCR法 ,对 36例胃癌患者的癌组织和相应的癌旁“正常”黏膜 ,以及 37例慢性胃炎患者的胃镜活检组织中 ,MAGE 1和MAGE 3基因的表达进行研究 ,并对RT PCR扩增产物中的目的基因片段进行DNA测序验证。结果 :36例胃癌组织中 ,MAGE 1基因的表达阳性率为 72 .2 2 % (2 6 / 36 ) ,MAGE 3基因的表达阳性率为 6 9.4 4 % (2 5 / 36 )。MAGE 1、MAGE 3的表达均为阳性者为 5 2 .78% (19/ 36 )。癌旁“正常”黏膜中 ,MAGE 1、MAGE 3基因的表达阳性率分别为 4 7.2 2 % (17/ 36 )和4 4 .4 4 % (16 / 36 ) ;MAGE 1、MAGE 3基因表达双阳性者为 30 .5 5 % (11/ 36 )。在 37例胃慢性炎症患者中 ,MAGE 1、MAGE 3基因的表达阳性率 ,分别为 2 9.73% (11/ 37)和 2 7.0 3% (10 /37) ;MAGE 1、MAGE 3基因的表达双阳性者为 8.1% (3/ 37)。胃癌组织中 ,MAGE 1和MAGE 3基因表达阳性率均高于癌旁和胃慢性炎症患者 ;在肿瘤组织中的表达阳性率与肿瘤浸润的深度和分化程度有关 (P <0 .0 5 ) ;而与临床病理分期及淋巴结转移无关 (P >0 .0 5 )。结论 :基于MAGE 1和MAGE 3基因在胃癌中的高表达率 ,可利用这两种蛋白作为靶?AIM: To detect the expression of melanoma antigen 1 and 3 genes (MAGE 1 and MAGE 3) and explore its significance in immunotherapatic application. METHODS: The expression of MAGE 1 and MAGE 3 in gastric cancer, para tumorous normal mucosa, and gastric biopsy tissues were detected by RT PCR. The target genes in RT PCR product were verified by means of DNA sequencing. RESULTS: MAGE 1 expression was positive in 26 out of 36 (72.22%) cases of gastric cancer, MAGE 3 positive in 25 out of 36(69.44%), and both MAGE 1 and MAGE 3 positive in 19 out of 36 (52.78%). Positive expression rates of MAGE 1, MAGE 3 and both MAGE 1 and MAGE 3 were 47.22%, 44.44% and 30.55%, respectively in para tumorous normal mucosa, while 29.73%, 27.03% and 8.1% in gastric biopsy tissues The positive rates of MAGE 1 and MAGE 3 gene expressions in tumor tissues was significant higher than those its parallelism mucosae and biopsy tissues from chronic gastritis. The expressions of MAGE 1 and MAGE 3 genes in tumor tissues were relevant to intruded depth and the differentiated degree of tumor cells ( P <0.05). CONCLUSION: MAGE 1 and MAGE 3 protein may be used as targets molecules of immunotherapy for gastric cancer and indexes for follow up study of chronic gastritis as well.

关 键 词：胃癌 慢性胃炎 MAGE-1 MAGE-3 基因表达 

分 类 号：R739.5[医药卫生—肿瘤]