冬虫夏草水提液对单个心室肌细胞钾通道的影响  

Effects of water extracts from codyceps sinensis on potassium current in isolatated ventricular cells

作　　者：王赫[1] 周宇宏[1] 单宏丽[1] 陈庆文[1] 张妍[1] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学药理学教研室

出　　处：《中国药理学通报》2004年第5期536-539,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金委员会生命科学部资助项目 ;No 3 0 2 715 99;国家自然基金资助项目 ;No 3 0 0 70 92 5;黑龙江省重大攻关项目 ;No2 0 0 10 10 10 0 1 0 0

摘　　要：目的　观察冬虫夏草水提液对豚鼠及大鼠心室肌细胞钾通道的作用 ,探讨冬虫夏草的抗心律失常作用机制。方法　应用全细胞膜片钳技术记录冬虫夏草水提物对豚鼠单个心室肌细胞内向整流钾电流 (IK1)、延迟整流钾电流 (IK)及大鼠心室肌细胞瞬时外向钾电流 (Ito)的影响。结果　应用 0 1g·L-1(生药浓度 )冬虫夏草水提液使豚鼠单个心室肌细胞内向整流钾电流在实验电压 - 12 0mV时从给药前(- 36 37± 5 15 ) pA/pF减少到 (- 2 9 70± 5 90 ) pA/ pF(n=5 ,P <0 0 5 ) ;延迟整流钾电流在实验电压 +70mV时 ,从给药前 (9 2 1± 2 4 2 ) pA/ pF增加至 (11 5 4± 2 98)pA/ pF(n =6 ,P <0 0 1) ;使大鼠心室肌细胞瞬时外向钾电流 (Ito)在实验电压 +5 0mV时 ,从给药前 (13 36± 0 88) pA/ pF增加至 (16 4 8± 1 0 9) (n =4 ,P <0 0 1)。结论　冬虫夏草抗心律失常作用与它对心肌细胞钾通道的作用有关。它增加IK,Ito的同时抑制IK1。AIM To investigate the antiarrhythmic mechanism of water extract from codyceps sinensis(CS) on transmembrane potassium currents in single ventricular myocyte of guinea pig and rat. METHODS Whole-cell patch-clamp was used to record inward rectifier K + current(I K1),delayed rectifier K + current (I K) and transient outward K + current (I to) in single ventricular myocyte of guinea pig and rat. RESULTS In ventricular myocytes of guinea pig, I K1 was decreased from (-36.37±5.15) pA/pF to (-29.70±5.90) pA/pF(n=5, P<0.05) following CS 0.1 g·L -1 at -120 mV; I k increased from (9.21±2.42) pA/pF to (11.54±2.98) pA/pF (n=6, P<0.01)at +70 mV. In rat, I to was increased from (13.36±0.88) pA/pF to (16.48±1.09)(n=4,P<0.01). CONCLUSION The antiarrythmic effects of codyceps sinensis may be related to potassium current. It increased I K and I to, decreased I K1, resulting in APD decrease without early and delayed afterdepolarization.

关 键 词：冬虫夏草 心律失常 离子通道 膜片钳技术 

分 类 号：R-332[医药卫生] R282.71