高浓度17β-雌二醇诱导巨噬细胞凋亡  

Apoptosis of macrophage induced by E_2 at high concentration

作　　者：洪敏[1] 孙小玉[1] 李晓冬[1] 朱荃[1] 

机构地区：[1]南京中医药大学规范化中药药理实验室,南京210029

出　　处：《中国药理学通报》2004年第5期568-571,共4页Chinese Pharmacological Bulletin

基　　金：国家"十五"攻关项目资助课题 ;No 2 0 0 1BA70 1A2 9

摘　　要：目的　考察高浓度 17β 雌二醇 (E2 )对巨噬细胞的影响。方法　用Hoechst332 5 8进行染色质荧光染色 ;扫描电子显微镜观察细胞形态 ;激光共聚焦显微镜检测胞质内钙离子浓度 [Ca2 + ]i 及线粒体膜电位。结果　荧光染色及扫描电镜显示E2 (≥ 1μmol·L-1)作用后巨噬细胞出现多种凋亡表现。E2 1μmol·L-1作用后 ,细胞 [Ca2 + ]i 明显升高 ,且有剧烈的波动。E2 1μmol·L-1使线粒体膜电位下降。结论　高浓度的E2 (≥ 1μmol·L-1)能诱导巨噬细胞的凋亡 ,并且与[Ca2 + ]iAIM To study the effect of 17β-estradiol(E 2) on apoptosis of macrophages at certain concentration.METHODS Fluorescent microscopy and scanning eletron microscopy(ESEM) were used to detect apoptosis changes of macrophages induced by E 2 at relatively high concentration and intracellular Ca 2+ and matochontrial transmembrane potential were examined by laser scanning confocal microscopy(LSCM). RESULTS Apoptosis of macrophages was induced by high concentration of E 2 (≥1 μmol·L -1). Intracellular Ca 2+ elevated and matochontrial transmembrane potential decreased after E 2 treatment. CONCLUSION Apoptosis of macrophages is induced by certain concentration of E 2 (≥1 μmol·L -1) and this may be related to elevated intracellular Ca 2+ and decreased matochontrial transmembrane potential.

关 键 词：17Β-雌二醇 凋亡 [Ca^2+] 线粒体膜电位 

分 类 号：R-332[医药卫生] R329.24