蜂毒素诱导骨肉瘤细胞U_2OS凋亡与坏死的实验研究  

Effect of melittin on apoptsis and necrosis of U_ 2OS cells

作　　者：陈永强[1] 朱振安[1] 郝永强[1] 戴尅戎[1] 张晨[2] 

机构地区：[1]上海第二医科大学附属第九人民医院骨科,上海200011 [2]第二军医大学附属长海医院中医科,上海200433

出　　处：《中西医结合学报》2004年第3期208-209,共2页Journal of Chinese Integrative Medicine

摘　　要：目的 :研究蜂毒素是否能够诱导骨肉瘤细胞U2 OS凋亡与坏死。方法 :U2 OS经不同浓度蜂毒素处理后 ,采用台盼蓝排染法测定细胞增殖抑制率 ,利用单克隆抗体 ,通过流式细胞仪检测AnnexinV、Apo2 .7及Fas蛋白的表达。结果 :6 4mg/L蜂毒素作用U2 OS 12h、2 4h ,其坏死率在 80 %以上。 16mg/L、32mg/L蜂毒素对细胞增殖有明显抑制作用 ,并且可诱导细胞凋亡和坏死的产生 ,增加细胞Aap2 .7及Fas蛋白的表达。结论 :蜂毒素高剂量有明显杀伤U2 OS的作用 ,低剂量具有促细胞凋亡作用 ,并能上调Fas蛋白的表达。Objective: To study the effect of melittin on apoptsis and necrosis of osteosarcoma cell line U 2OS in vitro. Methods: Osteosarcoma cell line U 2OS was treated with melittin. The growth and proliferation was observed by MTT assay and cell counting, and the necrosis was estimated by Trypan blue staining. The cell apoptsis,Fas and Apo2.7expression were detected by cytometer.Results: The data showed that melittin could inhibit the proliferation of U 2OS dose-dependently at 16 and 64 mg/L. Cell apoptsis was detected by cytometer, when the cells were treated by 16 mg/L and 32 mg/L of melittin respectively, and the percentages of Fas and Apo2.7 positive cells were increased. Conclusion: Melittin inhibits the proliferation of osterosarcoma cell line through up-regulating Fas expression and inducing apoptsis.

关 键 词：蜂毒素 骨肉瘤 肿瘤细胞 U2OS 细胞凋亡 细胞坏死 实验 

分 类 号：R738[医药卫生—肿瘤]