NS-398对人胃癌细胞株增殖及COX-2表达的影响  

The offect of NS-398 on the proliferatior of human gastric cancer cell line and COX-2 expression in vitro

作　　者：刘升云[1] 李建生[1] 张金平[1] 

机构地区：[1]郑州大学第一附属医院,河南郑州450052

出　　处：《山东医药》2004年第13期17-18,共2页Shandong Medical Journal

摘　　要：目的　体外观察选择性环氧化酶 2 (COX- 2 )抑制剂 NS- 398对人胃癌细胞株 SGC790 1细胞增殖及COX- 2表达的影响。方法　采用噻唑蓝 (MTT)法观察 NS- 398对 SGC790 1细胞增殖的影响 ,流式细胞仪(FCM)研究 NS- 398对 SGC790 1细胞凋亡的作用 ,免疫细胞化学观察 COX- 2蛋白的表达。结果　体外 NS- 398能减少 SGC790 1细胞株 COX- 2的表达 ,对 SGC790 1有细胞毒作用 ,可增加细胞凋亡率。结论　体外 NS- 398对SGC790 1细胞增殖有抑制作用。可能与抑制 COX-Objective To investigate the effect of a cyclooxygenase-2 inhibitor NS-398 on the proliferation of human gastric cancer cell line SGC7901 and influence on COX-2 expression in vitro.Methods Gastric cancer cell lines were treated with NS-398. Proliferation of the cells was detected by MTT assay. Apoptosis of cells was measured by flow cytometry (FCM), COX-2 protein expression was detected by immunocytochemistry.Results NS-398 suppressed proliferation of gastric cancer cell line SGC7901 and inhibited COX-2 expression. NS-398 had cytotoxic effect on the cell line and induced apoptosis in a dose and time dependent manner.Conclusion NS-398 inhibited SGC7901 gastric cancer cell growth in vitro, which may be explained by its inhibitory effect on COX-2 and induction of apoptosis.

关 键 词：NS-398 胃癌 增殖 COX-2 表达 

分 类 号：R735.2[医药卫生—肿瘤]