长江水系青鱼遗传多样性的研究  

Studies on the Genetic Diversity of Black Carp Populations in Yangze River

作　　者：方耀林[1] 余来宁[1] 许映芳[1] 姚雁鸿[1] 

机构地区：[1]中国水产科学研究院长江水产研究所,湖北荆州434000

出　　处：《湖北农学院学报》2004年第1期26-29,共4页Journal of Hubei Agricultural College

基　　金：国家"九五"科技攻关计划资助项目(96-008-01-03-01)

摘　　要：应用40个10碱基随机引物对长江干流金口和瑞昌江段的青鱼(Mylopharyngodonpiceus)群体以及长江水系的湘江青鱼群体进行随机扩增多态DNA(RAPD)分析,其中29个引物能扩增出1～10条清晰的RAPD带,有14个引物(占全部引物的35%)在青鱼不同个体的扩增产物中有多态性DNA片断。结果表明,青鱼金口、瑞昌、湘江3个群体的遗传相似度的平均值分别为0.9524、0.9612、0.9578,3个群体的Shannon遗传多样性值分别为0 2057、0.1483、0.1821。群体间的遗传相似度依次为金口-湘江:0.9487,瑞昌-湘江:0 9503,金口-瑞昌:0.9512,群体间的遗传相似度低于群体内的遗传相似度,遗传变异度则相反。3个群体的遗传分化指数为14.6%。Genetic analysis of three populations of black carp ( Mylopharyngodon piceus) from Jinkou,Ruichang and Xiangjiang within the middle reaches of the Yangtze River was conducted by using randomly amplified polymorphic DNA assay.Fourty 10-mer primers were used, of which, 11 produced nothing, and 29 did 1～10 DNA bands, respectively. Fourteen primers produced polymorphic DNA bands. Genetic analysis showed that the genetic similarity and the Shannon's index within Jinkou, Ruichang and Xiangjiang populations were 0.952 4,0.961 2 ,0.957 8 and 0.205 7,0.148 3,0182 1 separatively. The genetic similarity between populations of Jinkou-Xiangjiang, Ruichang-Xiangjiang and Jinkou-Ruichang were 0.948 7, 0.950 3 and 0.951 2 respectively. The genetic similarities in the three populations were higher than that between them. The coefficient of gene differentiation (Gst) was 14.6%.

关 键 词：长江水系 青鱼 遗传多样性 RAPD 遗传相似度 遗传变异度 遗传分化指数 

分 类 号：Q959.468[生物学—动物学] S965.111[农业科学—水产养殖]