烧伤后血清蛋白异常的进一步探讨  

Further study in the abnormal plasma proteins after burn injury

作　　者：黄文华[1] 胡祖祥[1] 罗中华 黎鳌[1] 

机构地区：[1]第三军医大学烧伤研究所,630038

出　　处：《中华整形烧伤外科杂志》1992年第2期89-92,T005,共5页

摘　　要：我们曾以十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS-PAGE),发现烧伤血清中至少有4个异常区带.我们对这些异常区带作了进一步分析,方法是用蛋白免疫印迹法,以12种抗人不同血清蛋白的抗体检测 SDS-PAGE 中的各区带,发现用抗触珠蛋白抗血清所着染的区带与 SDS-PAGE 中的异常区带非常符合。如果将烧伤血清先用离子交换层析分离,再检测所收集的各蛋白峰洗脱液对正常小鼠脾淋巴细胞是否具免疫抑制活性,则发现具免疫抑制活性的洗脱液均出现在第6蛋白峰.若将此具免疫抑制活性的洗脱液,再进行 SDS-PAGE 和免疫印迹分析,则用触珠蛋白抗血清检测所得区带,仍与烧伤血清中发现的异常区带相符.本实验结果显示在烧伤血清中可能有一类似触珠蛋白的物质具有免疫抑制活性.In our previous study at least 4 abnormal bands were found in burned sera when analyzed by SDS-PAGE.In this paper further exploration of the characteristics of these abnormalities of burned sera were presented.By way of western blotting,12 kinds of antisera againstt different human proteins as detecting antibodies were used.Results showed that bands deve- loped by anti-haptoglobin antiserum corresponded well with the abnormal bands in SDS-PAGE. (?)f the burned serum was separated by ion-exchange chromatography and its collected eluates containing protein peaks were tested for the immunosuppressive activities on normal murine spleen lymphocytes,the immunosuppressive eluates were usually found in the 6th protein peak. When these immunosuppressive eluates were further analyzed by SDS-PAGE and Western blotting,bands stained with anti-haptoglobin antiserum still coincided with the abnormal bands found in SDS-PAGE of burned serum.Result of this study suggests that one of the immunosuppressive factors found in burned serum may be a haptoglobin-like substance.

关 键 词：烧伤 血清蛋白 免疫抑制活性 

分 类 号：R644.03[医药卫生—外科学]