氯化汞对离体小鼠骨髓细胞和睾丸生殖细胞DNA的损伤作用  

Damaging effect of mercuric chloride on DNA of bone marrow cells and testicular cells in mice

作　　者：董杰影[1] 金龙金[1] 楼哲丰[1] 

机构地区：[1]温州医学院生物学实验教学中心,浙江温州325035

出　　处：《温州医学院学报》2004年第3期207-209,共3页Journal of Wenzhou Medical College

基　　金：温州市科技发展计划基金资助项目(S2002A018)

摘　　要：目的:探讨氯化汞对小鼠骨髓细胞和生殖细胞DNA的损伤作用。方法:利用彗星试验(SCGE)技术检测0.01、0.1、1.0 mmol/L氯化汞对离体小鼠骨髓细胞和睾丸细胞的DNA损伤。结果:0.01 mmol/L、0.1mmol/L、1.0 mmol/L氯化汞处理组骨髓细胞DNA损伤率分别为12.8％、57.2％和100％,与阴性对照组(5.5％)相比差异有显著性(P<0.01);睾丸细胞DNA损伤率分别为26.7％、45.5％和98.0％;这与阴性对照组(6.0％)相比差异有显著性(P<0.01)。其相应的彗星细胞DNA迁移长度分别为:骨髓细胞(20.38±2.98)、(51.72±5.15)、(92.91±3.28);睾丸细胞(19.29±1.63)、(27.77±3.01)、(66.31±3.11);分别与阴性对照组[(12.32±0.61),(15.15±1.35)]相比差异有显著(P<0.01)。结论:氯化汞可引起DNA链损伤,且损伤随着氯化汞剂量增加而加重。Objective: To study the damaging effect of mercuric chloride on DNA of bone marrow and testicular cells in mice.Methods:The damaging effects of 0.01, 0.1 and 1.0 mmol/L mercuric chloride on the bone marrow cells and the testicular cells in mice were detected with single cell get electrophorosis (SCGE) .Results:The damaged rates of DNA of bone marrow cells were 12.8% , 57.2% and 100% , respectively; and that of testicular cells were 26.7% , 45.5% and 98.0% , respectively. It had remarkable difference compared with the negative group of5.5% and 6.0%(P<0.01)The migration lengths of DNA of the relevant comet cell were: (20. 38 ± 2. 98) , (51.72 ± 5.15), (92.91 ± 3.73)in bone marrow cells and (19.29± 1.63), (27.77 ± 3.01) , (66.31 ± 3.11)in the testicular cells. It had remarkable difference compared with negative group of (12.32 ± 0.61) , (15.15 ± 1.35) (P< 0.05) .Conclusion:Mercuric chloride can induce breakage of DNA single strand and the damage aggravates with the increasing of the concentration of mercuric chloride.

关 键 词：氯化汞 骨髓细胞 生殖细胞 DNA损伤 彗星试验 小鼠 

分 类 号：X132[环境科学与工程—环境科学]