大鼠脑出血周边组织NO含量和NOS活性变化及对细胞凋亡的影响  

The effects of NO content and NOS activity on apoptosis around hematoma in acute intracerebral hemorrhage

作　　者：刘欣[1] 黄作义[1] 王复新[1] 

机构地区：[1]佳木斯大学附属第一医院神经内科,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2004年第3期1-3,共3页Heilongjiang Medicine and Pharmacy

摘　　要：目的 :研究大鼠脑出血周边组织一氧化氮 (N O)含量和一氧化氮合酶 (N OS)活性变化及与细胞凋亡的关系。方法 :1Wistar大鼠 10 4只 ,随机分为对照组、脑出血组、脑出血 +氮硝基左旋精氨酸 (N N L A)组 ,后两组各分为 (4 h、6 h、12 h、1d、3d、7d) 6个时间点。 2测定出血周边组织 N O含量、N OS活性及神经细胞凋亡。结果 :1大鼠脑出血周边组织 N O、诱导型一氧化氮合酶 (i N OS) ,4 h开始升高 ,3d i N OS、N O达峰值。 2大鼠脑出血周边组织 6 h出现凋亡 ,细胞 3d凋亡细胞达峰值 ,与i N OS峰值对应 ,7d时仍存在较多凋亡细胞。 3N N L A干预后 N O含量、i N OS活性及凋亡细胞数量与脑出血组对应时间点比较显著下降 ,差异显著。结论 :大鼠脑出血周边组织神经细胞存在长时间凋亡 ,N O、i N OS可以促进其凋亡 ,N OS抑制剂减少大鼠脑出血周边组织神经细胞凋亡。Objective: To study the dynamic changes in nitric oxide (NO) conten and nitric oxide synthase (NOS) activity and apoptosis around hematoma in acute intracerebral hemorrhage (ICH) and the relationship between them. Method: 104Wistar rats were randomly divided into control group, ICH group and ICH+NNLA group . The time (4h,6h,12h,1d,3d and 7d after ICH) was established for the latter two groups NO content, NOS activity and apoptosis around hematoma in ICH were determined.Results:①The NO content and NOS activity around hematoma in ICH began to rise at 4h and reached the top at about 3d .② The apoptosis around hematoma in ICH was detected at 6h, peaking at 3d .Until 7d there still was more apoptosis .③The NO content ,NOS activity and apoptosis significantly decreased at the same hour compared with the control after NNLA interference around hematoma in ICH . That difference was notable . Conclusion: The apoptosis lasts longer around hematoma in ICH . NO and NOS increase apoptosis and NOS inhibitor decreased it .

关 键 词：脑出血 细胞凋亡 一氧化氮 一氧化氮合酶 氮硝基左旋精氨酸(NNLA) 

分 类 号：R743.34[医药卫生—神经病学与精神病学]