紫杉醇诱导急性胰腺炎大鼠胰腺腺泡细胞凋亡机制的研究  

The mechanism by which taxol induces apoptosis of pancreatic acinar cells during pancreatitis in rats

作　　者：邵宏伟[1] 齐清会[1] 

机构地区：[1]天津医科大学总医院中西医结合外科,300052

出　　处：《中华普通外科杂志》2004年第3期163-165,共3页Chinese Journal of General Surgery

摘　　要：目的研究急性胰腺炎时紫杉醇诱导大鼠胰腺腺泡细胞凋亡的机制。方法利用胆胰管内注射脱氧胆酸钠的方法建立急性胰腺炎大鼠模型 ,并向腹腔内注射紫杉醇以诱导胰腺腺泡细胞凋亡。利用免疫组化法研究胰腺腺泡细胞凋亡相关蛋白Bcl 2、Bax、Fas、FasL及 p5 3表达的变化。结果急性胰腺炎时大鼠胰腺腺泡细胞中Bax(2 5 0 6± 94 2 )、Fas(2 79± 15 0 )和 p5 3(180± 5 6 )的表达增加 ,Bcl 2 (79± 4 2 )表达无明显变化 ,而FasL只表达于急性胰腺炎时胰腺间质浸润的炎性细胞中。结论急性胰腺炎时紫杉醇诱导的大鼠胰腺腺泡细胞凋亡与Bax、Fas/FasL系统以及p5 3蛋白有关。Objective To investigate the mechanism by which taxol induces apoptosis of pancreatic acinar cells during experimental acute pancreatitis in rats. Methods Intrapancreatobiliary duct injection of sodium deoxycholate was carried out to establish acute pancreatitis model in Wistar rats. Taxol was injected intraperitonealy to induce pancreatic acinar cell apoptosis. The expression of apoptosis associated protein Bcl-2,Bax,Fas,FasL and p53 was detected by immunohistochemistry. Results The expression of Bax(2 506±942),Fas(279±150) and p53(180±56) increased,while Bcl-2(79±42) remained unchanged in pancreatic acinar cells during acute pancreatitis,and the expression of FasL could only be detected in infiltrative inflammatory cells. Conclusions During acute pancreatitis,the acinar cell apoptosis induced by taxol is associated with activity regulation of Bax,Fas/FasL system and p53.

关 键 词：紫杉醇 急性胰腺炎 大鼠 胰腺腺泡细胞 细胞凋亡 P53 凋亡相关蛋白 

分 类 号：R576[医药卫生—消化系统] R979.1[医药卫生—内科学]