端粒酶与视网膜母细胞瘤组织病理学特征相关性的初步研究  

The study on the correlation between telomerase and histopathologic features of retinoblastoma

作　　者：任若瑾[1] 李彬[1] 孙宪丽[1] 郑邦和[1] 李辽青[1] 陈长喜[1] 

机构地区：[1]首都医科大学北京同仁眼科中心北京市眼科研究所病理室,100005

出　　处：《中华眼科杂志》2004年第4期234-238,共5页Chinese Journal of Ophthalmology

基　　金：首都医科大学基础-临床合作基金资助项目 (0 2JL14 )

摘　　要：目的　探讨端粒酶基因 (hTR)和端粒酶催化亚单位基因 (hTRT)在视网膜母细胞瘤 (RB)组织标本中的表达 ,研究端粒酶与RB增殖和分化的关系。方法　应用原位杂交方法检测hTR、hTRT在 37例RB和 2例正常组织标本中的表达 ;并对标本中的增殖细胞核抗原 (PCNA)进行免疫组化染色 ,经过量化 ,作为肿瘤的增殖指数 ;应用SPSS统计软件对各指标进行相关性分析。结果　RB标本中hTR和hTRT的阳性表达分别为 83 8%、89 2 % ,2例正常视网膜组织内的表达均为阴性。hTR、hTRT的表达水平与RB不同的增殖、分化程度的统计学差异均有显著意义 (均P <0 0 5 ) ,RB侵犯视神经情况越严重、分化程度越差 ,hTR、hTRT的表达越强。此外 ,RB的端粒酶hTR、hTRT指标间有一定相关性。结论　RB中端粒酶基因hTR和催化亚单位基因hTRT的高表达可作为判断RB恶性及其程度的新型标记物。hTR、hTRT与RB分化类型、视神经侵犯情况密切相关 ,为端粒酶表达水平反映RB分化程度和增殖能力提供理论依据。端粒酶在RB中的存在为开辟新型临床治疗方法 ,即通过抑制RB细胞端粒酶活性达到治疗目的提供了新思路。Objective To investigate the correlation between the expression of human telomerase RNA (hTR),human telomerase reverse transcriptase (hTRT) and the proliferation and differentiation of retinoblastoma (RB). Methods In 37 RB specimens,the expression of hTR and hTRT was investigated with the methods of in situ hybridization (ISH). Immunohistochemical method was performed to detect the expression of proliferating cell nuclear antigen (PCNA) in RB. All data were analyzed statistically with SPSS. Results Of 37 RB specimens,positive expression of hTR and hTRT was 83.8% and 89.2%,respectively,while negative results were observed in 2 normal retina tissues. The expressions of hTR and hTRT showed a close correlation to the proliferation and differentiation of RB. Furthermore,there was a close correlation among the expression of hTR and hTRT. Conclusions These results suggest that higher expression of hTRT and hTR may be used as new parameters for evaluating the degree of malignancy of RB. The close correlation between the expression of hTR and hTRT and proliferation and differentiation degrees of RB indicats that the expression of telomerase can influence the proliferation and differentiation of RB. Because the telomerase is presented in the Rb,therefore,modulating the telomerase activity may be used as a novel treatment for the RB. (Chin J Ophthalmol,2004,40:234-238)

关 键 词：端粒酶基因 视网膜母细胞瘤 组织病理学 端粒酶催化亚单位基因 原位杂交 检测 

分 类 号：R739.7[医药卫生—肿瘤]