高浓度胰岛素对兔视网膜Mller细胞表达血管内皮生长因子的影响  

Effects of high concentrations insulin on VEGF expression in cultured Müller cells

作　　者：宋鄂[1] 王越晖[2] 徐琪[3] 王心蕊[2] 朱向红[2] 吴家祥[2] 

机构地区：[1]吉林大学第一医院眼科,长春130021 [2]吉林大学基础医学院病理教研室,长春130021 [3]吉林大学基础医学院病理生理教研室,长春130021

出　　处：《中华眼科杂志》2004年第1期40-44,共5页Chinese Journal of Ophthalmology

基　　金：吉林省科学基金资助项目 ( 990 575 5);长春市科技基金资助项目 ( 0 1 1 0 6 530 )

摘　　要：目的　探讨高浓度胰岛素对体外培养的视网膜M櫣ｌｌｅｒ细胞表达血管内皮生长因子(VEGF)的影响。方法　采用免疫细胞化学法、原位杂交法及ELISA法 ,定性、定量测定在不同浓度胰岛素条件下 ,体外培养的兔视网膜M櫣ｌｌｅｒ细胞表达ＶＥＧＦ的变化?峁∫鹊核啬苊飨栽銮浚郑牛牵频谋泶?,并通过转录水平调节。结论　高浓度胰岛素可能通过刺激M櫣ｌｌｅｒ细胞编码ＶＥＧＦ基因的转录 ,增强VEGF蛋白的表达 ,从而加速糖尿病视网膜病变的新生血管形成。 (中华眼科杂志 ,2 0 0 4,40 :40 44)Objective To investigate the e ffects of high concentrations of insulin on the expression of vascular endothelial growth factor (VEGF) in cultured rabbit retinal Müller cells. Methods Immunocytochemistry, in situ hybridization and the ELISA method were used to study the effects of different concentrations of insulin on the expression of vascular endothelial growth factor (VEGF) in cultured rabbit retinal Müller cells qualitatively and quantitatively. Results VEGF expression was enhanced obviously by the insulin, which regulated the expression of VEGF at the transcription level. Conclusions VEGF expression in cultured Müller cells can be enhanced by high concentrations of insulin. Müller cells play a potential role in the development of neovascularization of diabetic retinopathy. (Chin J Ophthalmol, 2004, 40:40-44)

关 键 词：高浓度胰岛素 兔 视网膜 Mtiller细胞 血管内皮生长因子 新生血管形成 糖尿病 视网膜病变 

分 类 号：R774.1[医药卫生—眼科] R587.2[医药卫生—临床医学]