BRI基因的表达与非小细胞肺癌发生及转移潜能的关系  

Correlative study between expression of BRI gene and metastatic potential in human non-small cell lung cancer

作　　者：陈英准[1] 李钰[2] 邹嵘[2] 陈宇[2] 王艳颖[3] 冯会臣[2] 王吾如[4] 

机构地区：[1]哈尔滨医科大学附属第二医院病理科,150086 [2]哈尔滨医科大学基础医学院生物教研室,150086 [3]哈尔滨医科大学附属第三医院病理科,150086 [4]哈尔滨医科大学病理学教研室,150086

出　　处：《中华病理学杂志》2004年第1期62-66,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目(39970 396)

摘　　要：目的　探讨BRI基因在人非小细胞肺癌中的差异表达情况及其与肺癌发生及转移能力形成的相关性。方法　应用逆转录 聚合酶链反应 (RT PCR)、Northern印迹杂交方法分析BRI基因在一对遗传背景相同、转移能力不同的人肺腺癌细胞系AGZY83 a和Anip 973中的差异表达 ,并进一步检测BRI基因在另外 6个非小细胞肺癌细胞系 (SPC A 1,A549,95D ,TKB 18,GLC 82 ,PAa)及 3 0例临床肺癌标本中的表达情况。结果　BRI基因在 2细胞系中存在明显的差异表达 ,在具有高转移潜能的Anip973中高表达。在其他 6个细胞系中BRI的表达与肺癌转移潜能可能有关。同时发现与同一病例的正常肺组织相比 ,BRI基因在肺癌组织中高表达 ,其过表达在有淋巴结转移的肺癌为 6/ 8,无转移者为 45 5% (10 / 2 2 ) ,并且BRI基因在肺癌与正常肺组织之间存在转录本的差异。结论BRI基因在肺癌中表达上调 ,其 1Objective To investigate the potential relationship between BRI gene expression and metastatic potential in human non-small cell lung cancer(NSCLC). MethodsUsing semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and Northern blot hybridization techniques, differential expression of the BRI gene in human lung adenocarcinoma cell lines AGZY-83-a and Anip-973 was investigated. Having a much higher metastatic potential, Anip-973 was isolated from AGZY-83-a parental cell line. In addition, the other 6 non-small cell lungcancer cell lines (SPC-A-1,A549,95D,TKB-18,GLC-82,PAa)and 30 samples of lung cancer tissues with matched corresponding adjacent normal tissues were also analyzed. ResultsThere were significant differences in BRI gene expression between the two cell lines. BRI was preferentially expressed in Anip-973 cells compared to its parental cell line AGZY-83-a, and was also up-regulated in the other 6 lung cancer cell lines, correlating possibly with their metastatic potentials. BRI gene over-expression was observed in 30 lung cancer tissues compared with its corresponding adjacent normal tissues. A relative over-expression of BRI mRNA (tumor/normal≥2) was observed in 6 of 8 cancer samples with lymph node metastasis and 10 of 22(45.5%) samples without lymph node metastasis. Furthermore, two mRNA transcripts of BRI gene were observed: a 2.0 kb transcript which was mainly observed in normal lung tissues and a 1.6 kb transcript which was present as a dominant species in cancer tissues. ConclusionBRI mRNA expression is significantly up-regulated in NSCLC cell lines and clinical tumor samples. An alternatively spliced 1.6 kb mRNA is a major transcript of the gene in NSCLCs, suggesting that differential RNA processing and expression of BRI gene may play a role in the tumorigenesis and/or be related to the metastatic potential of human lung cancer.

关 键 词：BRI基因 基因表达 非小细胞肺癌 肿瘤转移 检测 

分 类 号：R734.2[医药卫生—肿瘤]