对脂多糖结合蛋白致炎的多肽序列分析  

Analysis of inflammatory peptide sequence of lipopolysaccharide-binding protein

作　　者：徐德斌[1] 钱桂生[1] 侯一峰[1] 赵哓利[1] 陈维中[1] 李淑萍[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所,重庆400037

出　　处：《重庆医学》2004年第5期718-720,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目 (30 1 70 366)

摘　　要：目的　应用噬菌体随机 1 2肽库探讨脂多糖结合蛋白 (LBP)致炎作用的多肽序列。方法　以LPS为目标分子 ,对噬菌体 1 2肽库进行亲合筛选 ,4轮筛选后 ,检测随机挑取的噬菌体克隆的结合活性和抑制活性 ,将得到的 1 6个可与LBP竞争性结合LPS的噬菌体克隆进行生物学功能检测 ,对获得的 9个阳性克隆进行测序 ,并与LBP序列比较。结果　 9个阳性克隆展示多肽的核心序列为WKXRKXFXKXXG ,与LBP的 91～ 1 0 2位氨基酸序列有较高同源性。结论　LBP的 91～ 1 0 2位氨基酸可能为其致炎作用部位。Objective To determine the inflammatory peptide sequence of lipopolysaccharide binding protein(LBP).Methods Using LPS as target,4 rounds of bio panning to a phage random 12 mer peptide library were carried out.One hundred clones were selected and used in binding test,competitive inhibition test and function test.Positive clones were sequenced and compared with LBP.Results Sixteen phage clones could inhibit the binding of LBP to LPS,and 9 of which could inhibit the function of LBP which enhanced the LPS dependent activation of PBMC.The core sequence of 9 positive clones was WKXRKXFXKXXG.By homology analysis,one higher homologous sequence was found in LBP 91 102 aa.Conclusion The 91 102 aa of LBP could be inflammatory peptide sequence.

关 键 词：噬菌体随机肽库 脂多糖结合蛋白 多肽 序列 

分 类 号：Q513[生物学—生物化学]