“彗星”分析法检测人癌裸鼠移植瘤的放射敏感性  

Comet assay study on the radiosensitivity of transplanted tumor models in nude mice

作　　者：高远红[1] 杨伟志[1] 闫洁[1] 袁智勇[1] 刘新帆[1] 徐国镇[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院放射治疗科

出　　处：《中华放射肿瘤学杂志》2004年第1期48-51,共4页Chinese Journal of Radiation Oncology

摘　　要：目的　探讨“彗星”分析法应用于人实体肿瘤放射敏感性检测的可能性。方法　应用“彗星”分析法 ,以尾力距之比 (RTM)作为终指标 ,检测人肺腺癌、人食管鳞癌和人鼻咽鳞癌等 3种人癌裸小鼠移植瘤的放射敏感性。裸小鼠移植瘤组织被消化并稀释成细胞浓度为 4× 10 4ml的单细胞悬液后 ,分成对照组 (0Gy)和不同剂量照射组 ,照射组分别给予 2、5、10和 15Gy的 6MVX射线的冰上照射 ,照射后立即进行“彗星”分析。结果　 3种人癌裸小鼠移植瘤细胞未照射时 (0Gy)的尾力矩 (TM)差异有显著性意义 (F =9.11,P <0 .0 1)。不同剂量 (2、5、10和 15Gy)照射后 ,3种人癌裸小鼠移植瘤细胞未做校正时的TM所反映放射敏感性由高到低的变化趋势依次为 :肺腺癌 >食管鳞癌 >鼻咽鳞癌 ,显然与临床一般印象不符 ;而经与对照组进行“本底”校正后的RTM所反映放射敏感性由高到低的变化趋势依次为 :食管鳞癌 >鼻咽鳞癌 >肺腺癌 ,则符合临床一般印象。结论　①应用“彗星”分析法检测实体肿瘤的放射敏感性 ,尾力矩必须进行“本底”校正 ,即扣除对照组本底误差后的尾力矩才能很好地反映实体肿瘤的放射敏感性差异。②“彗星”分析法可用于检测人体肿瘤组织的放射敏感性。ObjectiveTo evaluate the possibility of detecting human solid tumors radiosensitivity by comet assay. Methods The radiosensitivity of three human tumor xenografts (lung adenocarcinoma,esophageal squamous carcinoma and nasopharyngeal squamous carcinoma) were detected by comet assay with the R TM considered as the end point. Transplanted tumor specimens were taken and digested to single-cell suspensions with a cell concentration of 4×10 4 ml. For each xenograft,the resultant suspensions were divided into five groups and exposed to irradiation on ice to doses of 0 (control group),2,5,10 and 15 Gy,respectively. DNA damage was detected by comet assay immediately after irradiation. ResultsFor the unirradiated control group,the tail movement(TM) of the three xenografts showed significant differences(F=9.11,P<0.01). The tail movements in the three xenografts which had been exposed to irradiation (2,5,10 and 15 Gy) reflected the descending tendency of radiosensitivity as follows: lung adenocarcinoma>esophageal squamous carcinoma>nasopharyngeal squamous carcinoma,which is not consistent with the clinical observation. However,the descending trend of the relative radiosensitivity reflected by the adjusted tail moment (R TM ) was in the following sequences : esophageal squamous carcinoma > nasopharyngeal squamous carcinoma>lung adenocarcinoma; the esophageal squamous carcinoma was slightly more radiosensitive than nasopharyngeal squamous carcinoma (though without significant difference),which was exactly consistent with clinical observation. Conclusions 1. The tail movement should undergo background adjustment in order to reflect the difference of the radiosensitivity detected by comet assay in solid tumors. 2. Comet assay could be used for detecting the radiosensitivity of human solid tumors.

关 键 词：“彗星”分析法 检测 裸鼠 移植瘤 放射敏感性 食管鳞癌 鼻咽鳞癌 肺腺癌 

分 类 号：R73-3[医药卫生—肿瘤]