枯草杆菌电击法转化  

TRANSFORMATION OF BACILLUS SUBTILIS BY ELECTROPORATION

作　　者：周天鸿[1] 李月琴[1] 刘飞鹏[1] 温晋[1] 

机构地区：[1]暨南大学生物学系

出　　处：《暨南大学学报（自然科学与医学版）》1992年第1期65-69,共5页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：高电压电击法转化枯草杆菌,以菌株BR151和pBC11质粒为材料,获得每微克DNA的1.6×10~3个转化子。此方法主要依赖于电流脉冲的两个性质:电场强度和脉冲时间RC(时间常数)。Bacillus subtilis can be transformed with high efficiency by high voltage electroporation. 1.6×10~3 transformants/μg DNA were obtained with strain BR151 and plasmid pBC11. The process is highly dependent on two characteristics of the electrical pulse: the electric field strength and the pulse length RC(time constant). Procedure for high efficient electrotransformation of Bacillus subtilis is presented. Possible improvements are discussed.

关 键 词：电击转化 枯草杆菌 基因脉冲仪 

分 类 号：Q78[生物学—分子生物学]