抗HPV16E6核酶对宫颈癌CaSKi细胞侵袭表型和VEGF表达的影响  

Effects of anti-HPV16 E6-ribozyme on invasiveness of cervical carcinoma cell line CaSKi and vascular endothelial growth factor expression

作　　者：刘柯[1] 王丽莉[1] 郑燕芳[2] 张积仁[2] 

机构地区：[1]第一军医大学珠江医院妇产科,广东广州510282 [2]第一军医大学珠江医院肿瘤中心,广东广州510282

出　　处：《第一军医大学学报》2004年第5期529-532,共4页Journal of First Military Medical University

基　　金：广东省自然科学基金(96058)~~

摘　　要：目的探讨特异性抗HPV16E6核酶对宫颈癌CaSKi细胞侵袭表型和VEGF表达的影响。方法以脂质体法将抗HPV16E6核酶、空载体质粒分别导入CaSKi细胞,分别为CaSKi-R和CaSKi-P。测定CaSKi,CaSKi-R和CaSKi-P 3种细胞的生长曲线、软琼脂克隆形成率和裸鼠体内成瘤性,RT-PCR检测3种细胞中VEGF的表达。结果CaSKi、CaSKi-P细胞生长速率和软琼脂克隆形成率相近,CaSKi-R细胞的生长速度和软琼脂克隆形成率明显降低。CaSKi 、CaSKi-P致瘤性无显著差异,而CaSKi-R致瘤性显著低于CaSKi(P<0.05)。RT-PCR检测CaSKi-R细胞的VEGF mRNA的表达水平明显低于CaSKi、CaSKi-P细胞的表达,而后者的VEGF mRNA的表达水平相近。结论特异性抗HPV16E6核酶的导入降低了宫颈癌CaSKi细胞的增殖和侵袭表型,可能与宫颈癌CaSKi细胞内VEGF的表达下降有关。Objective To investigate the changes in the invasiveness of cervical cancer cell line CaSKi and the expression of vascular endothelial growth factor (VEGF) in response to treatment with anti-HPV16 E6-ribozyme, which plays a important role in the malignant phenotype and conversion of cervical cancer cells. Methods By means of lipofectin transfection, anti- HPV16 E6-ribozyme and empty eukaryotic expression plasmids were respectively transfected into CaSKi cell line (designated as CaSKi-R and CaSKi-P respectively). CaSKi, CaSKi-R and CaSKi-P cells were observed for their cell growth curves, clone forming ability on soft agar and tumorigenicity in nude mice. One-step reverse transcriptional PCR (RT-PCR) was employed to examine the expression of VEGF. Results No significant differences were found in the growth rate, clone forming ability and tumorigenicity between CaSKi and CaSKi-P cells. In contrast, CaSKi-R exhibited obviously decreased growth rate, clone forming ability and tumorigenicity (P<0.05). RT-PCR analysis showed that the expression levels of VEGF mRNA in CaSKi-R cells were lower than those in CaSKi-P and CaSKi cells. Conclusion Anti-HPV16 E6-ribozyme may reduce the proliferative ability and invasiveness of cervical cancer cell line CaSKi, possibly through decreasing VEGF expression in CaSKi cells.

关 键 词：抗HPV16E6核酶 宫颈癌 CaSKi细胞侵袭表型 VEGF 表达 

分 类 号：R737.33[医药卫生—肿瘤]