应用cDNA微阵列技术研究EB病毒LMP1介导的鼻咽癌中转移相关基因的表达  

Study on Expression of Metastasis-associated Genes in Nasopharyngeal Carcinoma Cell Line Mediated by EBV-LMP1 with cDNA Microarray

作　　者：曾亮[1] 刘轶平[1] 赵燕[1] 唐发清[1] 石颖[1] 李嵬[1] 曹亚[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所

出　　处：《病毒学报》2004年第1期17-21,共5页Chinese Journal of Virology

基　　金：国家重点基础发展规划 ( 973 )"恶性肿瘤侵袭和转移的机理及分子阻遏" ( 2 0 0 2CB5 13 10 0 ) ;国家自然科学基金( 3 0 10 0 0 0 5 )

摘　　要：探讨EB病毒基因组编码的癌蛋白LMP1对鼻咽癌细胞中转移相关基因表达的影响。采用蛋白质印迹法检测在强力霉素 (Dox)诱导下 ,鼻咽癌细胞系 pTet on LMP1HNE2 (L7细胞 )中LMP1表达的时效和量效关系。应用cDNA微阵列技术建立诱导性LMP1介导鼻咽癌细胞中转移相关基因差异表达谱 ;运用RT PCR验证cDNA微阵列筛选差异基因表达的可靠性。与LMP1不表达的L7细胞比较 ,LMP1高表达的L7细胞中 7个基因的表达显著上调 ,1 2个基因的表达显著下调。随机选择其中 4个基因进行RT PCR ,结果显示 ,这些基因表达阳性 ,且与微阵列中的变化趋势一致。LMP1可能通过激活和This paper was to investigate the effect of EB virus encoded latent membrane protein 1(LMP1) on expression of metastasis associated genes in nasopharyngeal carcinoma.Time dependent and dose dependent correlation between Dox and inducible LMP1 were confirmed with Western blotting,differential display was established to study the metastasis associated genes induced by LMP1 in nasopharyngeal carcinoma cell lines with cDNA microarray technique.Results of cDNA micrarray was confirmed by RT PCR.Compared to uninduced LMP1 negative NPC cell lines,7 genes were up regulated and 12 genes were down regulated by high expressed LMP1,changes of expression of 4 genes selected at random for RT PCR were consistant with results from cDNA microarray.It is possible for LMP1 to activate or inhibit expressions of metastasis associated genes,thus to be involvd in metastasis of nasopharyngeal carcinoma cells.

关 键 词：潜伏膜蛋白1 EB病毒 CDNA微阵列 鼻咽癌 转移 

分 类 号：R739.63[医药卫生—肿瘤] R373[医药卫生—临床医学]