含外源类蜗牛毒素基因的AcMNPV的虫体感染性研究  

Study of Foreign Baculoviral ctl Gene by Overexpression Using AcMNPV Bacmid System in vivo

作　　者：卢玉蓉[1] 冯枞棣[1] 吴东[1] 孙修炼[1] 邓菲[1] 袁丽[1] J.M.Vlak 胡志红1 

机构地区：[1]中国科学院武汉病毒研究所分子病毒学重点实验室和无脊椎动物病毒学联合开放实验室,湖北武汉430071 [2]DepartmentofVirology

出　　处：《中国病毒学》2004年第3期276-280,共5页Virologica Sinica

基　　金：国家杰出青年基金(3002503);中国科学院创新工程重要方向性项目(KSCX2-1-02KSCX-SW-301-09);863项目(2001AA214031);973项目(2003CB114202)

摘　　要：类蜗牛毒素基因(conotoxinlike,ctl)是在一些杆状病毒基因组中存在的与蜗牛毒素类似的一类基因,其功能尚不清楚。本文利用苜蓿银纹夜蛾核多角病毒(AcMNPV)bacmid表达系统构建了含油桐尺蠖核多角体病毒(BusuNPV)ctl基因的重组病毒AcBac-ph-ctl。在细胞水平上对ctl基因的RT-PCR分析表明,该基因转录出mRNA。在甜菜夜蛾体内进行了生物活性测定,结果表明AcBac-ph-ctl与对照野生型AcMNPV的LC50,ST50无显著性差异,表明在此系统中,外源的CTL无杀虫增效性能。The conotoxinlike (ctl) gene is a small gene in the genomes of several baculoviruses. The biological function of baculoviral ctl gene remains unknown. In this report, we generated a recombinant AcMNPV(AcBac-ph-ctl) which contains the ctl gene from BusuNPV using AcMNPV bacmid system. The transcription of ctl gene by AcBac-ph-ctl was confirmed by RT-PCR in infected Sf21 cells. To study the CTL function in vivo, the recombinant viruses were used to infect Spodotera exigua peros, and the infectivity and action speed were determined. No significant difference between the median lethal concentration (LC50) and the median survival time (ST50) of the wild type virus AcMNPV and the recombinant virus was found .

关 键 词：类蜗牛毒素基因(ctl) ACMNPV BACMID LC50 ST50 转录 生物测定 

分 类 号：Q78[生物学—分子生物学]