云南高校图书馆联盟文献共享服务平台- BACMID

BACMID: 作品数：11被引量：12H指数：2; 导出分析报告; 相关领域：农业科学生物学更多>>; 相关作者：类承凤刘柳周吟吴柳柳苏松坤更多>>; 相关机构：中国科学院浙江大学西北农林科技大学华中师范大学更多>>; 相关期刊：《Science China(Life Sciences)》《Virologica Sinica》《Agricultural Sciences》《中国免疫学杂志》更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划中国科学院知识创新工程重要方向项目更多>>

Construction and Characterization of a Novel Bacmid AcBac-Syn Based on a Synthesized Baculovirus Genome被引量：1: 《Virologica Sinica》2021年第6期1566-1574,共9页Yu Shang Hengrui Hu Xi Wang Hualin Wang Fei Deng Manli Wang Zhihong Hu; This work was supported by the grants from the National Science Foundation of China(Grant Nos.31800143 and 31872640);the Key Research Program of Frontier Sciences of the Chinese Academy of Sciences(Grant No.QYZDJ-SSW-SMC021);the Hubei Provincial Innovation Center of Agricultural Sciences and Technology(Grant No.2019-620-000-001-017)。; Baculoviruses are large DNA viruses which have been widely used as expression vectors and biological insecticides.Homologous recombination and Bac-to-Bac system have been the main methods for manipulating the baculovi...; 关键词：Synthetic genome BACULOVIRUS Autographa californica multiple nucleopolyhedrovirus(AcMNPV) Large DNA virus

Recombinant expression and purification of functional vascular endothelial growth factor-121 in the baculovirus expression system被引量：3: 《Asian Pacific Journal of Tropical Medicine》2016年第12期1170-1174,共5页Nastaran Mohseni Ali Jahanian Najafabadi Fateme Kazemi-Lomedasht Roghaye Arezomand Mahdi Habibi-Anbouhi Delavar Shahbazzadeh Mahdi Behdani; supported financially by Iran National Science Foundation(INSF);grant number 91004026; Objective: To express human Vascular endothelial growth factor121(VEGF121) in insect cells. Methods: A gene construct containing VEGF was cloned in the p Fast Bac-HTA vector, followed by transformation in DH10 BAC. Th...; 关键词：Vascular endothelial growth factor Baculovirus expression system Recombinant bacmid

潜伏: 《电子显微学报》2016年第5期408-408,共1页陈德蕙; 拍摄时间：2000年10月18日;样品类型：体外培养昆虫细胞; 制作方法：常规电镜超薄切片方法;仪器型号：Philips CM120 作品描述：多年前曾应用透射电镜观察按常规方法制作的体外培养细胞超薄切片样品中检出经重组Bacmid转染的昆虫细胞...; 关键词：电镜超薄切片仪器型号昆虫细胞 BACMID 体外培养病毒粒子芽生内嵴杆状病毒细胞线粒体

Expression and localization of the spore wall protein SWP26 of <i>Nosema bombycis </i>in the silkworm BmN cell line被引量：2: 《Agricultural Sciences》2013年第2期79-84,共6页Feng Zhu Zhongyuan Shen Shengyan Xiao Yajie Yue Xuliang Fu Xudong Tang Li Xu Xijie Guo; The microsporidian spore wall proteins, as the main components of the spore wall, play a key role in spore adherence to host cells and in recognition of the parasite by the host during the invasion process. In this st...; 关键词：BACMID EXPRESSION MICROSPORIDIA NOSEMA BOMBYCIS Spore Wall Protein

重组杆状病毒Bacmid-HER2胞外段基因的构建及鉴定: 《细胞与分子免疫学杂志》2007年第12期1183-1185,共3页陈亚静魏映辉吴秀丽王莉王丽颖于永利; 国家高技术研究发展计划(863)资助项目(2002AA214141); 目的:克隆人表皮生长因子受体2胞外段基因(HER2-ECD)的cDNA,构建重组杆状病毒Bacmid-HER2-ECD。方法:从Her2高表达的人乳腺癌细胞系SK-BR-3中提取总RNA,用逆转录-聚合酶链反应(RT-PCR)扩增HER2ECD基因cDNA。利用T/A克隆连接法,首先构建p...; 关键词：HER2ECD 基因克隆昆虫杆状病毒表达系统

Tn7-mediated Introduction of DNA into Bacmid-cloned Pseudorabies Virus Genome for Rapid Construction of Recombinant Viruses: 《中国病毒学》2007年第4期316-325,共10页Fang-fang ZHUAN Zhen-feng ZHANG Di-ping XU Yan-hong SI Han-Zhong WANG Ghopur MIJIT; Key technologies R&D program (2006BAD06A01) from the Ministry of Science and Technology of China.; lacZα-mini-attTn7 was inserted into the intergenic region between the gG and gD genes in a PRV bacterial artificial chromosome (BAC) by homologous recombination in E. coli. The resulting recombinant BAC (pBeckerZF1) ...; 关键词：狂犬病病毒无性系 DNA转导 Tn7转座子

中华蜜蜂(Apis cerana cerana) MRJP1基因Bacmid表达载体的构建: 《蜜蜂杂志》2007年第S1期47-51,共5页陶挺苏松坤陈盛禄杜宏沪; 蜂王浆(RJ,Royal Jelly)是哺育蜂咽下腺与上颚腺分泌的供蜂王和3日龄以内蜜蜂幼虫食用的浆状物质。蛋白质是蜂王浆的重要活性组分,蜂王浆主蛋白MRJPs(Major royal jelly proteins)具有多种生物活性。以中华蜜蜂为材料,通过引物设计和PC...; 关键词：中华蜜蜂蜂王浆 MRJP1 BACMID 表达载体

含外源类蜗牛毒素基因的AcMNPV的虫体感染性研究被引量：2: 《中国病毒学》2004年第3期276-280,共5页卢玉蓉冯枞棣吴东孙修炼邓菲袁丽 J.M.Vlak 胡志红1; 国家杰出青年基金(3002503);中国科学院创新工程重要方向性项目(KSCX2-1-02KSCX-SW-301-09);863项目(2001AA214031);973项目(2003CB114202); 类蜗牛毒素基因(conotoxinlike,ctl)是在一些杆状病毒基因组中存在的与蜗牛毒素类似的一类基因,其功能尚不清楚。本文利用苜蓿银纹夜蛾核多角病毒(AcMNPV)bacmid表达系统构建了含油桐尺蠖核多角体病毒(BusuNPV)ctl基因的重组病毒AcBac-p...; 关键词：类蜗牛毒素基因(ctl) ACMNPV BACMID LC50 ST50 转录生物测定

以Bacmid-杆状病毒系统表达人CTLA4胞外区蛋白的研究: 《第三军医大学学报》2003年第21期1899-1901,共3页贺伟峰易绍萱陈希炜吴军; 第三军医大学附属西南医院院管课题(2002)~~; 目的在Bacmid-杆状病毒-昆虫细胞系统中表达人CTLA4。方法采用PCR技术,自pUC19-CTLA4Ig质粒中获取人CTLA4胞外区cDNA,将其克隆入pFastBacl质粒中,经DNA自动测序仪进行DNA序列鉴定,并进一步将其转座入Bacmid中,在昆虫细胞HzAM1中进行表...; 关键词：CTLA4 杆状病毒 Hz细胞 BACMID

An efficient method of constructing homologous recom binant baculovirus with PCR-amplified fragments被引量：1: 《Science China(Life Sciences)》2003年第4期431-437,共7页侯松旺陈新文王汉中胡志红; supported partly by the National Natural Science Foundation of China(Grant Nos.30025003&30070034);the Hundreds-Talent Program and Knowledge Innovation Program(Grant No.kscx2-1-02,kscx2-SW-301-09)of the Chinese Academy of Sciences.; This paper describes a rapid method of constructing homologous recombinant baculovirus in E. coli with PCR-amplified fragments. By using this method, the traditional steps of constructing transfer vector are omitted. ...; 关键词：phage l red system baculovirus Bacmid gene replacement gene deletion HASNPV recombinant virus.

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