广州地区106例汉族人群MICA和MICB微卫星基因座单体型研究  

The Study on the Haplotype of MICA and MICB Microsatellite Locus In Guangzhou Han Population

作　　者：冯明亮[1] 张景怡[1] 谢军华[1] 陈亮[1] 陆琼[1] 郭晓俊[1] 杨剑豪[1] 季芸[1] 

机构地区：[1]上海市血液中心,上海200051

出　　处：《遗传》2004年第3期291-294,共4页Hereditas（Beijing)

基　　金：上海市卫生局资助项目(98408)~~

摘　　要：利用聚合酶链反应和荧光(6-FAM)自动化检测技术对广东地区汉族106例无亲缘关系样本进行MICA基因外显子5和MICB基因内含子1微卫星基因座多态性及其单体型分布调查。根据群体资料估算两者间的单体型频率、连锁不平衡参数、相对连锁不平衡参数。结果显示,广州地区汉族人群MICA和MICB微卫星基因座基因型分布符合Hardy-Weinberg平衡法则,共检出MICA微卫星基因座5个等位基因,MICB微卫星基因座14个等位基因。其中MICA A5基因频率最高(0.2877),A4基因频率最低(0.1321)。MICB CA14等位基因频率最高(0.3255),CA19、CA28等位基因频率最低(0.0047),未检出CA27。21种MICA-MICB单体型频率大于1％(连锁不平衡参数>0),其中单体型A5-CA14(16.73％),A5.1-CA18(8.75％),A4-CA26(3.76％),A9-CA15(3.66％)和A6-CA21(2.61％)为强连锁常见单体型(x^2>3.84,P<0.05)。This study is to investigate genetic polymorphisms and haplotypes of microsatellite locus in the exon 5 of the MICA gene and intron 1 of the MICB gene based on 106 samples of Guangzhou Han Population by polymerase chain reaction and fluorescent technique (6-FAM). The corresponding haplotype frequencies, linkage disequilibria values and relative linkage disequilibria values were estimated based on population data. The results show that the genotype distributions of MICA and MICB microsatellite meet Hardy-Weinberg equilibrium in Guangdong Han pop- ulation. In total, 5 alleles of MICA microsatellite locus and 14 alleles of MICB microsatellite locus were observed. MICA A5 was the most common allele (0. 2877) , whereas A4 was the least popular one (0. 1321 ). MICB CA14 was the most common allele (0. 3255) , and CA19 and CA28 were the least popular ones (0. 0047 ). CA27 was not observed. Twenty-one kinds of MICA-MICB haplotypes occurred at frequencies of more than 1% (linkage disequi libria value > 0 ). The common MICA-MICB haplotypes were A5-CA14 (16.73% ), A5.1-CA18 (8.75 % ), A4- CA26 (3.76% ),A9-CA15 (3.66%) and A6-CA21 (2.61% ) (x^2> 3.84, P<0.05), and they were strong linkage disequilibria.

关 键 词：广州地区汉族 MICA MICB 微卫星基因座 单体型 

分 类 号：Q987[生物学—遗传学]