双链DNA直接测序法在环境诱变剂致突变分子机制研究中的应用Ⅱ.甲基丙烯酸环氧丙酯诱导质粒抗性基因突变的测定  

APPLICATION OF DIRECTLY SEQUENCING OF DOUBLE STRANDED DNA IN RESEARCH OF EXPERIMENTAL MUTAGENS II.DETERMINATION OF MUTATED PLASMID PBR322 DRUG RESISTANT GENE INDUCED BY GLYCIDYL METHACRYLATE

作　　者：高惠兰[1] 谢大英[1] 左瑾[2] 方福德[2] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,北京100050 [2]中国医学科学院基础医学研究所,北京100005

出　　处：《癌变．畸变．突变》1993年第1期26-28,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：运用我所建立的双链DNA直接测序法测定了GMA诱导的质粒抗性基因片段(Tc^R)EcnRI/SaII片段(～0.3Kb)的序列,并与相应正常基因片段序列作一比较,所得结果具有一定意义。本实验表明:双链DNA直接测序法可应用于环境诱变剂研究。The EcoRI/SalI fragment (~0.3Kb) from mutant ApRTcS of plasmid pBR322 induced by glycidyl methacrylate (GMA) has been sequenced after recom-binated with vector using directly sequencing for double stranded DNA. In most case the mutation occured in the form of deletion and insertion of cytidine and guanine to the sequenced fragment.The results showed that the method directly sequencing for double stranded DNA is useful to the study of environmental mutagens.

关 键 词：双链DNA 直接测序 环境 诱变剂 GMA 

分 类 号：R994.6[医药卫生—毒理学]