Aβ_(42)及其亚单位疫苗接种正常成年大鼠的行为学观察  

Observation of Cognitive Behavior After Complete and Subunit of Aβ_(42) Vaccine Inoculation in Normal Adult Rats

作　　者：林贤[1] 胡金家[1] 汪华侨[1] 姚志彬[1] 

机构地区：[1]中山大学中山医学院脑研究室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2004年第3期208-211,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金重点资助项目(20013137);中山市科技局重点攻关项目 (4209606)

摘　　要：【目的】 探讨Aβ42及其亚单位肽疫苗接种正常成年大鼠后对其认知行为的影响?【方法】 采用固相肽合成法和肽疫苗技术制备Aβ42 N端1～15和C端36～42亚单位片段疫苗和Aβ42全肽疫苗? ①Aβ42及C端亚单位肽疫苗接种:32只SPF级成年雄性SD大鼠随机分为MAP36～42组,牛血清白蛋白组(BSA组),Aβ42组和对照组,每组8只?②Aβ42亚单位片段多联疫苗接种:同样条件的SD大鼠随机分为MAP1～15组,多联疫苗组Ⅰ,多联疫苗组Ⅱ和对照组?应用Morris水迷宫检测免疫接种后大鼠的认知行为能力?【结果】 SD大鼠接种Aβ42及其亚单位疫苗后,其平均逃避潜伏期以及平台象限的游泳距离的百分比,与对照组大鼠的比较,差异均无显著性(P >0.05)? 【结论】 Aβ42及其亚单位肽疫苗接种后对正常成年SD大鼠的认知行为无影响?Objective To investigate the behavioral variation of normal adult rats after they were inoculated by complete and subunit of Aβ42 vaccine. Methods We constructed entire peptide and subunit peptide vaccine of Aβ42 by means of Fmoc SPPS(solid phase peptide synthesis) and peptide vaccine technique. ①In the inoculation of complete and C-terminal subunit vaccine of Aβ42, 32 adult male Sprague-Dawley rats (SPF grade) were divided into MAP36～42 group, BSA group, Aβ42 group and control group (PBS group). ②In the inoculation of multivalent vaccine of Aβ42, another 32 same species of rats were also divided into MAP1～15 group, multivalent vaccine groupⅠ, multivalent vaccine groupⅡ and control group. After finishing all inoculations, the cognitive behavior of SD rats was as-sessed by Morris water maze Results Not only the average latencies but also the percentage of to-tal swimming distance in the platform quadrant showed no significant discrepancy between the experimen-tal groups and control group (P< 0.05). Conclusion There are no behavioral variation of the SD rats after they were inoculated by completer and subunit of Aβ42 vaccine.

关 键 词：Β淀粉样蛋白 亚单位肽疫苗 水迷宫 免疫接种 认知行为 阿耳茨海默病 

分 类 号：R749.16[医药卫生—神经病学与精神病学]