多囊卵巢综合征患者血浆TNFα、sTNFR2水平与胰岛素抵抗的相关性  

Relationships Between TNFα,sTNFR2 and Insulin Resistance in Polycystic Ovary Syndrome

作　　者：王竹晨[1] 顾熊飞[2] 杨冬梓[1] 邝健全[1] 

机构地区：[1]中山大学附属第二医院妇产科 [2]中山大学生物化学教研室广东广州510080

出　　处：《中山大学学报（医学科学版）》2004年第3期268-271,280,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省卫生厅科研基金资助项目(2001189)

摘　　要：【目的】 探讨血浆肿瘤坏死因子α(TNFα)?可溶性肿瘤坏死因子受体Ⅱ(sTNFR2)与多囊卵巢综合征(PCOS)患者胰岛素抵抗和血清雄激素(T)水平的相互关系?【方法】 PCOS患者和对照者根据体重指数(BMI)分为PCOS肥胖组(21例)?PCOS非肥胖组(25例)? 肥胖对照组和非肥胖对照组,各25例,采用ELISA法测定血浆TNFα ?sTNFR2水平,化学发光法测定血清性激素6项,已糖激酶终点法测定血清葡萄糖浓度,时间分辨免疫分析法测定血清胰岛素浓度,按Cederholm的方法计算胰岛素敏感指数(ISI)?【结果】 ①PCOS肥胖组血浆TNFα和sTNFR2水平及PCOS非肥胖组血浆sTNFR2水平均较非肥胖对照组明显升高,且以肥胖者升高更为明显?②血浆TNFα与其ISI在PCOS肥胖组和肥胖对照组均呈负相关(P< 0.05)?③血浆sTNFR2与其ISI在PCOS肥胖组?PCOS非肥胖组和肥胖对照组均呈负相关(P< 0.05)?【结论】 PCOS肥胖组血浆TNFα和sTNFR2水平及PCOS非肥胖组血浆sTNFR2水平均较非肥胖对照组明显升高,且与其ISI呈负相关,提示二者可能与PCOS患者发生胰岛素抵抗有关?Objective To investigate the relationships between plasma tumor necrosis factor al-pha(TNFα), soluble tumor necrosis factor receptor Ⅱ (sTNFR2) and insulin resistance (IR) and serum testosterone (T) in women with polycystic ovary syndrome (PCOS) . Methods Patients with PCOS and healthy controls were divided into the obese PCOS group (21 cases) , the non-obese PCOS goup, the obese control group and the non-obese control group (25 case) accord-ing to their body mass index (BMI). There were 25 cases in each group. The levels of TNFα and sTNFR2 in plasma were determined by ELISA, and the concentrations of insulin and sexual hormones in serum were assayed by chemiluminescent method and radioimmuno analysis respective-ly.Insulin sensitivity index (ISI) was calculated by Cederholm method. Results ① The levels of TNFα and sTNFR2 in the obese PCOS group and the level of sTNFR2 in the non-obese PCOS group were markedly higher than that in the non-obese control group. This disorder was quite serious in the obese PCOS women (P< 0.05). ② There were negative correlation be-tween TNFα and ISI in the obese PCOS group (P< 0.05) and the obese control group (P< 0.05). ③ There were negative correlation between sTNFR2 and ISI in the obese PCOS group (P< 0.05), the non obese PCOS group (P< 0.05) and the obese control group (P< 0.05). Conclusions The levels of TNFα and sTNFR2 in plasma in the obese PCOS group and the level of sTNFR2 in plasma in the non obese PCOS group increase markedly than the non-obese control group, and are negatively correlated with their ISI. It suggests that TNFα and sTNF2 might be the potential etiologic factors for IR in PCOS.

关 键 词：多囊卵巢综合征 血浆 TNFΑ 细胞因子 STNFR2 胰岛素抵抗 相关性 ELISA法 

分 类 号：R711.75[医药卫生—妇产科学]