低氧性肺动脉高压大鼠肺组织硫化氢的变化  

Changes of hydrogen sulfide in lung tissue of rat with hypoxic hypertention

作　　者：张春雨[1] 杜军保[1] 卜定方[2] 闫辉[1] 斯琴[3] 唐朝枢[4] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学第一医院中心实验室,北京100034 [3]中国医学科学院基础医学研究所病理生理教研室,北京100005 [4]北京大学第一医院心血管病研究所,北京100034

出　　处：《基础医学与临床》2004年第2期145-148,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金 (30 0 70 796 ) ;国家重点基础性研究项目 (G2 0 0 0 5 6 90 5 ) ;北京市自然科学基金 (70 1 2 0 2 1 ) ;985北京大学心血管重大研究项目

摘　　要：本文旨在探讨低氧对大鼠内源性硫化氢体系的变化。采用生化反应方法测定血浆中硫化氢的含量和肺组织中硫化氢合酶的活性 ,采用定量竞争性RT PCR的方法检测肺组织中胱硫醚 γ 裂解酶 (CSE)mRNA的含量。结果显示 ,与对照组相比 ,低氧组大鼠的血浆硫化氢含量明显减少 [(1 92 2± 2 2 1 ) μmol Lvs (30 1 6± 32 4 ) μmol L ,P <0 0 1 ) ],肺组织中硫化氢合酶的活性明显下降 [(0 1 2 7± 0 0 2 3)vs (0 2 78± 0 0 99)nmol mgwettissue·min ,P <0 0 1 ],CSEmR NA的含量明显减少 [(1 0 2± 0 1 5 )× 1 0 - 6 fmolvs (2 1 7± 0 2 2 )× 1 0 - 6 fmol,P <0 0 1 ]。以上研究表明 。To investigate the impact of hypoxia on endogenous hydrogen sulfide (H 2 S) system in rats lung tissue. Plasma level of H 2 S and H 2 S generating enzymes activity in homogenates of rat lung tissue was measured by biochemical methods. Cystathionine γ lyase (CSE) mRNA in lung tissues was determined by Quantitative Competitive RT PCR. The results showed that plasma level of H 2 S, the enzymatic activity of H 2 S generating enzymes and the amount of CSE mRNA in lung homogenates of rats in hypoxia group were all significantly decreased as compared with those in control group [(192 2±22 1)μmol/L vs (301 6±32 4)μmol/L, (0 127±0 023 ) vs (0 278±0 099)nmol/mg wet tissue·min, (1 02±0 15)×10 -6 fmol vs (2 17±0 22)×10 -6 fmol, respectively, all P <0 01]。These results suggested that the endogenous H 2 S system of rats was suppressed by hypoxia.

关 键 词：低氧 肺动脉高压 大鼠 肺组织 硫化氢 

分 类 号：R361[医药卫生—病理学]