Effects of cg,t11-conjugated linoleic acid on adhesion of human gastric carcinoma cell line SGC-7901  

作　　者：Bing-QingChen Yan-MeiYang QiWang Yan-HuiGao Jia-RenLiu Jing-ShuZhang Xuan-LinWang Rui-HaiLiu 

机构地区：[1]DepartmentofNutritionandFoodHygiene,PublicHealthCollege,HarbinMedicalUniversity,Harbin150001,HeilongjiangProvince,China [2]MedicalCollegeofShantouUniversity,Shantou515031,GuangdongProvince,China [3]ChineseCenterforDiseaseControlandPrevention,theCenterforEndemicDiseaseControl,Beijing.China [4]FoodScienceandToxicology,DepartmentofFoodScience,108StockingHall,CornellUniversity,Ithaca,NY14853-7201,USA

出　　处：《World Journal of Gastroenterology》2004年第10期1392-1396,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.30070658

摘　　要：AIM: To investigate the effect of c9, t11-conjugated linoleic acid (c9,t11-CLA) on the adhesion of human gastric carcinoma cell line (SGC-7901).METHODS: SGC-7901 cells were at first treated with different concentrations (25, 50, 100, 200 μmol/L) of c9,t111-CLA and 1 mL/L ethanol (as a negative control) for 24 h.Using adhesion assay and Western blot, we investigated the ability of SGC-7901 cells to adhere to intracellular matrix and examined the expression of E-cadherin (ECD), α-catenin,intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in these cells.RESULTS: The attachment rate to laminin of SGC-7901 cells treated with different concentrations of c9,t11-CLA (0,25, 50, 100, and 200 μmol/L) was 100.0±3.3, 95.7±4.0,89.2±4.6, 87.9±6.1, and 65.9±5.8, respectively. The attachment rate to fibronectin was 100.0±4.7, 96.8±3.8,94.5±4.1, 76.5±4.3, and 61.8±4.8, respectively. The attachment rate to Matrigel was 99.9±6.6, 91.4±6.8,85.5±7.4, 79.3±5.6, and 69.6±5.1, respectively. Besides,c9,t11-CLA could increase the level of ECD and α-catenin,and decrease the level of ICAM-1 and VCAM-1 in SGC-7901 cells.CONCLUSION: c9, t11-CLA can reduce the adhesion of human gastric carcinoma cells to laminin, fibronectin and Matrigel. c9,t11-CLA can increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in human gastric carcinoma cells.AIM:To investigate the effect of c9,t11-conjugated linoleic acid (c9,t11-CLA) on the adhesion of human gastric carcinoma cell line (SGC-7901). METHODS:SGC-7901 cells were at first treated with different concentrations (25,50,100,200μmol/L) of c9, t11-CLA and 1mL/L ethanol (as a negative control) for 24h. Using adhesion assay and Western blot,we investigated the ability of SGC-7901 cells to adhere to intracellular matrix and examined the expression of E-cadherin (ECD),α-catenin, intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in these cells. RESULTS:The attachment rate to laminin of SGC-7901 cells treated with different concentrations of c9,t11-CLA (0, 25,50,100,and 200μmol/L) was 100.0±3.3,95.7±4.0, 89.2±4.6,87.9±6.1,and 65.9±5.8,respectively.The attachment rate to fibronectin was 100.0±4.7,96.8±3.8, 94.5±4.1,76.5±4.3,and 61.8±4.8,respectively.The attachment rate to Matrigel was 99.9±6.6,91.4±6.8, 85.5±7.4,79.3±5.6,and 69.6±5.1,respectively.Besides, c9,t11-CLA could increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in SGC- 7901 cells. CONCLUSION:cg,t11-CLA can reduce the adhesion of human gastric carcinoma cells to laminin,fibronectin and Matrigel.c9,t11-CLA can increase the level of ECD and α- catenin,and decrease the level of ICAM-1 and VCAM-1 in human gastric carcinoma cells.

关 键 词：胃癌 细胞培养 亚油酸 SGC-7901细胞系 细胞粘附作用 

分 类 号：R735.2[医药卫生—肿瘤]