PTEN基因转染的胶质瘤细胞对γ刀照射敏感性的变化  

Sensitization of glioma cells transfected with wild-type PTEN gene to γ knife

作　　者：陈宏颉[1] 杨卫忠[2] 石松生[2] 林毅兴[2] 王锐[2] 

机构地区：[1]南京军区福州总医院神经外科,福州350001 [2]福建省协和医院神经外科,福州350001

出　　处：《第二军医大学学报》2004年第5期503-506,共4页Academic Journal of Second Military Medical University

基　　金：福建省教委科研基金(K2001083).

摘　　要：目的:观察转入正常PTEN基因的胶质瘤细胞经γ刀照射后生长能力及黏附力的改变,探索PTEN基因对胶质瘤细胞放射敏感性的影响。方法:以RT-PCR方法体外扩增PTEN全长基因,与质粒pcDNA3.1连接,构建重组真核载体pcD-NA3.1-PTEN,并用之转染PTEN失活的U251细胞(人胶质母细胞瘤细胞系),用48 h的半数致死剂量(中心剂量和周边剂量分别为9.00和4.50 Gy)进行γ刀等中心照射,并采用MTT法和纤维粘连蛋白黏附实验观察照射前后细胞的生长及黏附力改变。结果:空载体转染组与未转染组照射前后细胞存活率和黏附率均无显著性差异,而PTEN基因转染的细胞存活率及黏附率在照射前后均显著低于空载体转染组及未转染组(P<0.01),且照射后下降程度更加明显。结论:PTEN基因转染的胶质瘤细胞对于γ刀照射的敏感性提高。Objective:To study the sensitization of gliomas cells tansfected with wild-type PTEN gene to 7 knife. Methods : PTEN gene cDNA was amplified by RT-PCR. A recombinant plasmid pcDNA3. 1-PTEN was constructed by cloning PTEN gene into pcDNA3. 1. U251 cells were all transfected with wild-type PTEN plasmid vector (pcDNA3. 1-PTEN). Then the pcDNA3. 1-PTEN-transfected cells,untransfected cells and pcDNA3. 1-transfected cells were all treated by γ knife. The growth ability and adhesion of those cells were observed. Results:The survival rate and adhesion rate of cells transfected with pcDNA3. 1-PTEN decreased significantly compared with those untransfected or transfected with pcDNA3. 1(P<0. 01),while there was no significant difference between the last 2 groups. Conclusion:Wild-type PTEN transfection may have a positive impact on the γ knife therapy of gliomas.

关 键 词：PTEN基因转染 胶质瘤 γ刀照射敏感性 黏附力 侵袭力 

分 类 号：R730.264[医药卫生—肿瘤]