B_(16)-黑色素瘤实验性肝转移方法的建立  

A Novel Approach to the Generation of Experimental Hepatic Metastases in Murine Model System

作　　者：黄曙云 姚立人[1] 张载福[1] 谢克平[1] 荣桂芳 

机构地区：[1]安徽医科大学病理生理教研室

出　　处：《安徽医科大学学报》1993年第1期13-15,共3页Acta Universitatis Medicinalis Anhui

摘　　要：经C_(57)BL/6小鼠脾内注射同系B_(16)—黑色素瘤细胞,建立实验性肝转移模型。经脾脏(腹外法)直接注射0.1ml瘤细胞悬液(含2×10~6个瘤细胞),肿瘤细胞经脾静脉进入门静脉而停驻于肝脏,18d后肝转移瘤发生率为70%。与经典的大容积脾内注射法(注射量为1ml瘤细胞悬液,1min后切脾)相比,本法保留了脾脏,有更好的临床相关性,从而提供了一种良好的实验性肝转移模型。An experimental model of hepatic metastasis was developed by injection of B-16 melanoma single tumor cell suspension via spleen. Tumor cells flowed through the splenic vein into the portal vein and lodged in the liver. Laparotomy and splenectomty were not performed, When inoculum doses was 2×10~6 cells per mouse, the hepatic metastases rate was 70% after 18d. The model gives some analogy to clinical hepatic metastases and reqires simple procedure. The model provides a useful tool for the study of hepatic metastases.

关 键 词：肝肿瘤 肿瘤转移 黑色素瘤 

分 类 号：R73－37[医药卫生—肿瘤]