分化诱导剂对脊索瘤细胞体外粘附、移动和侵袭的作用  

Study on effects of differentiation inducer on adhesion, movement and invasion of chordoma cell line in vitro

作　　者：王育才[1] 范清宇[1] 张殿忠[1] 单乐群[1] 尹艳[1] 

机构地区：[1]第四军医大学唐都医院骨科,陕西西安710038

出　　处：《第四军医大学学报》2004年第10期904-905,共2页Journal of the Fourth Military Medical University

摘　　要：目的 :探讨分化诱导剂六亚甲基二乙酰胺 (hex aethylenebisacetamide,HMBA)对人脊索瘤CM 31 9细胞体外粘附、移动和侵袭的作用 .方法 :用 2mmol/LHMBA体外诱导培养CM 31 9细胞 ;用MTT(thiazalylblue,噻唑蓝 )比色法测定细胞在重构基底膜Matrigel上的粘附 ;用MillicellPCF培养系统测定细胞的移动性和侵袭性 .结果 :分化诱导剂HMBA诱导的CM 31 9细胞在 30min和 90min粘附实验中的粘附率 ,分别下降了 2 7%和 31 % .分化诱导剂HMBA对CM 31 9细胞体外移动和侵袭的抑制率分别为 6 5 .5 0 %和 6 9.85 % .结论 :分化诱导剂HMBA对人脊索瘤CM 31 9细胞具有抗侵袭作用 .AIM: To explore the effects of differentiation inducer (HMBA) on the adhesion, movement and invasion of chordoma cell line (CM 319) in vitro . METHODS: CM 319 cell lines were induced by HMBA (2 mmol/L) in vitro , the adhesion of the cells was detected by MTT and the movement and invasion of the cells were detected by Millicell PCF culture system. RESULTS: The adhesion rate of the cell line induced by HMBA reduced 27% and 31%, respectively after 30 min and 90 min culture in vitro . The suppression rate of movement and invasion of CM 319 induced by HMBA was 65.5% and 69.85%, respectively. CONCLUSION: HMBA has some anti invasion effects on the human chordoma cell line (CM 319).

关 键 词：脊索瘤 细胞系 分化诱导剂 侵袭性 

分 类 号：R738.1[医药卫生—肿瘤]