N-糖链合成相关β-1,4-半乳糖基转移酶在大鼠坐骨神经损伤后的表达变化  

CHANGES OF GENE EXPRESSION OF β-1,4-GALACTOSYLTRANSFERASE Ⅰ, Ⅱ, Ⅴ IN INJURED RAT SCIATIC NERVES

作　　者：沈爱国[1] 严美娟[1] 龚蕾蕾[1] 关晓颖[1] 程纯[1] 顾建新[2] 

机构地区：[1]南通医学院江苏省神经再生重点实验室,南通226001 [2]复旦大学上海医学院基因研究中心,上海200032

出　　处：《神经解剖学杂志》2004年第3期220-224,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金 ( No.3 0 3 0 0 0 99);江苏省自然科学基金 ( BK2 0 0 3 0 3 5 );江苏省高校自然科学研究计划 ( 0 3 KJB180 10 9)资助项目

摘　　要：为了分析与 N-糖链合成相关β-1,4-半乳糖基转移酶 - 、 、 (β-1,4-galactosyltransferase , , ,β-1,4-Gal T- , , ) m RNA在正常和损伤坐骨神经组织的表达变化。本研究采用 RT-PCR方法 ,分析 β-1,4-Gal T- 、 、 在小鼠坐骨神经中的表达存在。以扩增的 c DNA片断标记探针 ,Northern blot分析β-1,4-Gal T- 、 、 m RNA在正常和损伤大鼠坐骨神经的表达变化。结果表明 :通过 RT-PCR方法 ,检测到 β-1,4-Gal T- 、 、 在小鼠坐骨神经中的表达存在 ;采用 Northern blot方法 ,发现β-1,4-Gal T- 、 、 在正常大鼠坐骨神经中有表达 ,并在坐骨神经损伤后发生表达变化 ,但三种同功酶的表达变化各不相同。结论 :本实验发现 β-1,4-Gal T- 、 、 在坐骨神经有表达 ,并在坐骨神经损伤后发生表达变化。提示与The expression changes of β-1,4-galactosyltransferase Ⅰ, Ⅱ and Ⅴ(β-1,4-GalT-Ⅰ, Ⅱ and Ⅴ) which were associated with synthesizing N-linked oligosaccharides in normal and injured rat sciatic nerves were analyzed in the present study. The fragments of β-1,4-GalT-Ⅰ, Ⅱ and Ⅴ were obtained by RT-PCR through total RNA of mice sciatic nerves. Amplified cDNA fragments were prepared for the probes to analyze the expression changes of β-1,4-GalT-Ⅰ, Ⅱ and Ⅴ in normal and injured rat sciatic nerves by Northern blot. β-1,4-GalT-Ⅰ, Ⅱ and Ⅴ mRNA were expressed in mice sciatic nerves. The expression of three β-1,4-GalT was changed individually in rat injured sciatic nerves. The expression changes of β-1,4-GalT-Ⅰ, Ⅱ and Ⅴ in normal and injured rat sciatic nerves suggest that N-linked oligosaccharides may play important roles in maintaining normal peripheral nerve function and promoting injured nerve regeneration.

关 键 词：大鼠 坐骨神经 RT-PCR Β-1 4-半乳糖基转移酶 表达 蛋白修饰 

分 类 号：R363[医药卫生—病理学]