慢性复合应激对大鼠学习与记忆及海马NMDA受体亚基NR2A和NR2B表达的影响  

THE EFFECTS OF CHRONIC MULTIPLE STRESS ON LEARNING AND MEMORY AND NMDA RECEPTOR SUBUNITS NR2A AND NR2B EXPRESSION IN THE HIPPOCAMPUS OF RATS

作　　者：程少容[1] 刘能保[1] 张敏海[1] 刘少纯[1] 李辉[1] 孙臣友[1] 刘向前[1] 汪薇曦[1] 

机构地区：[1]华中科技大学同济医学院解剖学系组织胚胎学教研室,武汉430031

出　　处：《神经解剖学杂志》2004年第3期275-280,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金 ( No. 3 0 2 70 44 6)资助项目

摘　　要：为了观察慢性复合应激对大鼠学习与记忆的影响和海马内 NMDA受体亚基 NR2 A、NR2 B表达的变化。本研究将成年雄性 Wistar大鼠分为实验组和对照组 ,实验组动物每天交替暴露于复合应激原环境中达 6周 ,用 Morris水迷宫和 Y迷宫作业测试其空间学习与记忆成绩 ,再采用免疫组织化学和图像处理方法分析海马 CA1 、CA3、齿状回区的 NR2 A和 NR2 B的表达。结果显示 :( 1) Morris水迷宫测试 :慢性复合应激组大鼠寻找平台的潜伏期较对照组明显缩短 ,Y迷宫测试 :慢性复合应激组大鼠学会躲避电击的正确次数较对照组明显增多 ;( 2 )慢性复合应激组海马内 NMDA受体亚基 NR2 B表达水平较对照组明显上调 ,NR2 A表达水平无显著变化。结论 :慢性复合应激可增强学习与记忆能力 。To study the effects of chronic multiple stress on learning and memory and NMDA receptor subunits NR2A and NR2B expression in hippocampus of rats, Wistar male rats were randomly divided into control group and experimental group, the rats in the experimental group were alternatively exposed to the situation of chronic multiple stress for 6 weeks. After completion of the stress for experimental rats, both groups of rats were tested by Morris water maze and followed by Y-maze. Afterwards, the brain tissue sections were performed by immunohistochemistry to demonstrate the expression of NR2A and NR2B and immunoreactivity in the hippocampal CA 1, CA 3 and dentate gyrus was analyzed by computer-assisted image analysis system. The results showed that: (1) by comparing with the rats of control group, the latent periods to search platform was significantly shortened in experimental rats; and in the test of Y-maze, the time to correctly avoid the electric stroke was significantly increased in the experimental rats; (2) by comparing with the control group, the expression of NR2B was significantly up-regulated, the expression of NR2A was not significantly changed. The results indicate that chronic multiple stress might increase the ability of learning and memory and one of these mechanisms might be related to up-regulation of expression change of NMDA receptor subunit NR2B.

关 键 词：应激 大鼠 学习 记忆 海马 NMDA受体 NR2A NR2B 表达 N-甲基-D-天冬氨酸受体 

分 类 号：R363[医药卫生—病理学]