重症肌无力患者造血干/祖细胞及其亚群富集纯化的研究  

Collection and purification of myasthenia gravis CD34 + hematopoictic stem (progenitor) cells and its sup-groups

作　　者：秦超[1] 莫雪安[2] 陆锐[2] 凌莉[2] 莫武宁[2] 孙圣刚[1] 童萼塘[1] 

机构地区：[1]华中科技大学同济医学院协和医院神经内科,430022 [2]广西医科大学第一附属医院

出　　处：《脑与神经疾病杂志》2004年第3期205-206,共2页Journal of Brain and Nervous Diseases

基　　金：广西科技攻关项目资助(桂科攻0322025-9)

摘　　要：目的:获得高纯度造血干/祖细胞及其亚群,用于重症肌无力(MG)造血干细胞移植治疗和生物学特性的研究。方法:用免疫磁珠法(Mini MACS)从MG骨髓中富集CD 34+细胞后再用荧光激活细胞分选(Fluorescent activatedcell sorter,rAGS)纯化CD34+/CD38+和T CD34+/CD38-细胞亚群。结果:MiniMACS分选的CD34+细胞群纯度达90％,FACS分选的CD34+/CD38-和CD34+/CD38+两细胞亚群纯度可达95％以上。结论:联合使用MiniMACS和FACS可迅速大量纯化HSC/HPC及其重要细胞亚群,可用于临床造血干细胞移植、基因治疗和研究HSC/HPC及其亚群生物学特性。Objective: To collect and purify the bone marrow CD34 + hematopofetic stem celts and its sub-groups trom the patients with myasthenia gravis. Methods: CD34 + cells were collected and purified by means of imunomagnetic beads on MiniMACS device. Flow cytometry was emplyed to determine percentage of CD34 + cells, CD34 + /CD38 cells and CD34 + /CD38 + cells before and after miniMACS. And to detect cell ability after CD34 + cell purification, Results: The purity of CD34 + cells a-mount to more than 90% after, the purification. The purity of CD34 + /CD38- cells and CD34 + /CD38 + cells amount to more than 95% after FACS. Conclusions: The Conbination of MiniMACS and FACS can sort effectively the bone marrow CD34 + cells and its sub-groups so that the bone marrow CD34 + cells and its sub-groups are used for hematopoietic stem cell transplantation, gene therapy and for study of HSC/HPC and its cell sub-groups.

关 键 词：造血干/祖细胞 免疫磁珠法 流式细胞计量术 重症肌无力 

分 类 号：R7461[医药卫生—神经病学与精神病学]