The Inhibitory Effect of Somatostatin Analogue RC-160 on the Growth of Endometrial Carcinoma Cell Line HEC-1A  

作　　者：LiangRui TangXiaohui ZhuGaixia GaoYane GuoYin 

机构地区：[1]DepartmentofGynecology＆Obstetrics,the2ndHospitalofXi＇anJiaotongUniversity,Xi＇an [2]DepartmentofGynecology＆Obstetrics,theAffiliatedHospitalofTokyoUniversity,Japan

出　　处：《现代妇产科进展》2004年第3期238-240,共3页Progress in Obstetrics and Gynecology

摘　　要：Objective:To evaluate the inhibitory effect of somatostatin analogue RC-160 on the growth of human endometrial cancer cells (HEC-1A) in vitro.Method:RT-PCR was used to examine the existence of somatostatin receptors on the HEC-1A cells. The anti-proliferative effect of RC-160 on the growth of HEC-1A cells was detected by using BrdU incorporation test.TUNEL staining was used to find out whether apoptosis was involved in the inhibitory process.Result:All the five somatostatin receptor subtypes were demonstrated in HEC-1A cells.RC-160 reduced the HEC-1A cell growth stimulated by serum in a dose-dependent manner.The effect was maximal at the concentration of 10 -5 M after 48 hours’ treatment.No apoptosis was detected.Conclusion:Somatostatin analogue RC-160 can inhibit the proliferation of endometrial carcinoma cell line HEC-1A through binding to the somatostatin receptors on the cells.It seems that apoptosis is not mainly responsible for the inhibition.Objective:To evaluate the inhibitory effect of somatostatin analogue RC-160 on the growth of human endometrial cancer cells (HEC-1A) in vitro.Method:RT-PCR was used to examine the existence of somatostatin receptors on the HEC-1A cells. The anti-proliferative effect of RC-160 on the growth of HEC-1A cells was detected by using BrdU incorporation test.TUNEL staining was used to find out whether apoptosis was involved in the inhibitory process.Result:All the five somatostatin receptor subtypes were demonstrated in HEC-1A cells.RC-160 reduced the HEC-1A cell growth stimulated by serum in a dose-dependent manner.The effect was maximal at the concentration of 10 -5 M after 48 hours' treatment.No apoptosis was detected.Conclusion:Somatostatin analogue RC-160 can inhibit the proliferation of endometrial carcinoma cell line HEC-1A through binding to the somatostatin receptors on the cells.It seems that apoptosis is not mainly responsible for the inhibition.

关 键 词：HEC-1A 子宫内膜癌 癌细胞 RC-160 生长抑制素 

分 类 号：R737.33[医药卫生—肿瘤]