汉防己甲素联合屈洛昔芬逆转K562/ A02细胞耐药与诱导凋亡相关性的研究  

Reversal Effect of Tetrandrine in Combination with Droloxifene on Cell Line K562/A02 and Its Correlation with Inducted Apoptosis

作　　者：周云[1] 陈宝安[1] 董颖[1] 张鹏[1] 程坚[1] 盛茗[1] 王婷[1] 高峰[1] 

机构地区：[1]东南大学医学院附属中大医院血液科,南京210009

出　　处：《中国实验血液学杂志》2004年第3期321-323,共3页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号 3 9970 83 2

摘　　要：本研究旨在探讨汉防己甲素 (tetrandrine,Tet)联合屈洛昔芬 (droloxifene ,DRL)对耐药细胞系K5 6 2 A0 2的逆转作用与诱导凋亡有无相关性。采用AnnexinV PI法测定耐药调节剂汉防己甲素、屈洛昔芬单独或联合应用后细胞凋亡的情况。结果发现 0 .6 2 μg ml汉防己甲素或 1.94 μg ml屈洛昔芬单独作用于K5 6 2细胞 4 8小时后均可诱导一定比例的细胞凋亡 ,作用呈时间依赖性 ,两者联合应用作用增强。 0 .6 2 μg ml汉防己甲素或 1.94 μg ml屈洛昔芬单独作用于K5 6 2 A0 2细胞均不能诱导细胞凋亡 ,两者联合应用 72小时可诱导小部分细胞凋亡。结论 :汉防己甲素 ,屈洛昔芬逆转耐药的机理与诱导K5 6 2 A0 2细胞凋亡无关。To study the correlation of the reversal effect of tetrandrine (Tet) in combination with droloxifene (DRL) on multidrug resistant cell line K562/A02 and induction apoptosis,the apoptosis of K562 cells and K562/A02 cells after the treatment of Tet and DRL alone or their combination was detected by flow cytometry (FCM). The results showed 0 62 μg/ml Tet and 1.94 μg/ml DRL alone could induce apoptosis of K562 cells in time-dependent manner after culture for 48 hours. Neither Tet at 0.62 μg/ml nor DRL at 1.94 μg/ml alone could induce apoptosis of K562/A02 cells,but in the conbination of them two induced apoptosis of few K562/A02 cells in culture for 72 hours. In conclusion,the mechanism of Tet and DRL reversing multidrug resistance has no correlation with the apoptosis of K562/A02 cells.

关 键 词：多药耐药性 汉防己甲素 屈洛昔芬 K562细胞 A02细胞 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]