亚硝酸钠和多聚磷酸钠对日本沼虾的毒性研究  

Toxicity of Nitrite-Na and Sodium Polyphosphate to the Shrimp Macrobrachium nipponense

作　　者：李志华[1] 谢松[1] 王维娜[1] 王军霞[1] 

机构地区：[1]河北大学生命科学学院,保定071002

出　　处：《动物学杂志》2004年第3期12-16,共5页Chinese Journal of Zoology

基　　金：河北省教育厅资助项目 (No .2 0 0 2 2 73)

摘　　要：针对目前危害虾类养殖的水污染中比较严重的亚硝酸盐和多聚磷酸盐污染 ,主要研究亚硝酸钠和多聚磷酸钠对日本沼虾的毒性影响。通过实验得出 :亚硝酸钠对日本沼虾 2 4、48、96h的半致死浓度分别为 46、2 6、1 3 3 3mg L ,多聚磷酸钠对日本沼虾 2 4、48、96h的半致死浓度分别为 1 2 3 3 3 3、1 1 80、1 0 80mg L。实验表明 ,活性氧是随着亚硝酸钠和多聚磷酸钠的浓度增加而增加 ,而超氧化物歧化酶的酶活力则是随着亚硝酸钠和多聚磷酸钠的浓度增加先稍微增加 ,后又下降 ,这表明虾的免疫系统对低浓度的毒物有一定的抵抗能力 ,但随着毒物浓度增大 ,虾的免疫系统会受到抑制 ,虾的免疫功能下降 ,抗病力降低 。The pollution of nitrite and sodium polyphosphate is a serious problem at present.The main aim of this study is to reveal the toxicity of nitrite-Na and sodium polyphosphate to the shrimp Macrobrachium nipponense. Results showed that the LC 50 of nitrite-Na to the shrimp M.nipponense is 46,26 and 13^33 mg/L,and the LC 50 of sodium polyphosphate to the shrimp is 1?233^3,1?180 and 1?080 mg/L at 24,48 and 96?h,respectively.Results also showed that the quantity of ROS was also enhanced with the increase in the concentration of nitrite-Na and sodium polyphosphate,while the activity of superoxide dismitase was slightly increased but then decreased significantly.The results suggest that the immune system of the shrimp M.nipponense has some resistance to the low concentration of toxicants.However,as the concentration of toxicants increases,the immune system is prohibited,leading to death of the shrimp M.nipponense.

关 键 词：日本沼虾 亚硝酸钠 多聚磷酸钠 半致死 超氧阴离子 超氧化物歧化酶 

分 类 号：S945.1[农业科学—水产养殖]