检测CK19 mRNA、CEA mRNA诊断非小细胞肺癌外周血微转移  

Detection of CK19 and CEA mRNA expression for the diagnosis of peripheral blood micrometastases in patients with non-small cell lung cancer

作　　者：朱广迎[1] 刘德林[1] 王绪[1] 陈杰[1] 

机构地区：[1]徐州医学院肿瘤研究所放疗科

出　　处：《中国肺癌杂志》2004年第3期226-229,共4页Chinese Journal of Lung Cancer

基　　金：江苏省卫生厅科研 (Z970 9)资助~~

摘　　要：目的　探讨检测细胞角蛋白 (cytokeratin 19,CK19)mRNA、癌胚抗原 (cancerembryonicantigen ,CEA)mRNA诊断非小细胞肺癌 (non smallcelllungcancer ,NSCLC)患者外周血微转移的临床意义。 方法　应用RT PCR法检测 3 5例NSCLC患者外周血中的CK19mRNA、CEAmRNA ,并以 2 0例良性病变、2 0例健康人作对照。结果　 3 5例NSCLC患者外周血CK19mRNA、CEAmRNA阳性表达率分别为 40 %和 5 7%,两者均阳性者 40 %,两者均阴性者 43 %;肺良性疾病患者 2 0例 ,CK19mRNA阳性 2例 ( 10 %) ,无CEAmRNA阳性者 ;2 0例健康对照 2个指标检测结果均为阴性。随访发现CK 19mRNA阳性、CEAmRNA阳性和二者均阴性患者的远处转移率分别为 5 0 %、5 6%和 2 7%。结论　CEAmRNA和CK19mRNA是检测肺癌患者外周血微转移的良好标志物 。Objective To explore the sensitivity and clinical significance of detection of CK19 mRNA and CEA mRNA expression for the diagnosis of micrometastases in the peripheral blood of patients with non small cell lung cancer (NSCLC). Methods Nested reverse transcription polymerase chain reaction (RT PCR) was used to detect CK19 mRNA and CEA mRNA in the peripheral blood of 35 patients with NSCLC. Twenty patients with pulmonary benign lesions and 20 normal healthy volunteers were served as controls.Results The positive rates of CK19 mRNA and CEA mRNA for NSCLC patients were 40% (14/35) and 57% (20/35) respectively, 14 cases (40%) were positive for both mRNA, and 15 cases (43%) were both negative for both mRNA. No blood samples from patients with pulmonary benign lesions was positive for CEA mRNA, but 2 samples (10%) were positive for CK19 mRNA. Of these 20 samples from normal volunteers, none was positive for both CK19 mRNA and CEA mRNA. The distant metastasis rate of patients with positive CK 19 mRNA , positive CEA mRNA and both negative in the peripheral blood were 50%, 56% and 27% respectively. Conclusion Both CK 19 mRNA and CEA mRNA are good markers to detect micrometastasis in the peripheral blood of patients with NSCLC. Detection of CK19 and CEA mRNA expression might be helpful to predict the prognosis of patients with lung cancer.

关 键 词：RT-PCR 肺肿瘤 微转移 CEA MRNA CK19 MRNA 

分 类 号：R734.2[医药卫生—肿瘤]