人IFN-β基因脂质体转染胶质瘤细胞及对其增殖的抑制作用  

Growth inhibition of glioma cell line SHG44 by transfection of human interferon-β gene liposome

作　　者：林伟[1] 章翔[1] 王占祥[1] 郭衍[1] 张剑宁[1] 费舟[1] 王西玲[1] 梁景文[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710033

出　　处：《中华神经外科疾病研究杂志》2004年第3期226-229,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：全军医药卫生科研基金资助项目 (0 1MA1 87)

摘　　要：目的　观察β 干扰素 (IFN β)基因脂质体pSV2IFN β转染人胶质瘤细胞系SHG4 4及其对SHG4 4细胞增殖的影响。方法　应用流式细胞仪、酶联免疫吸附试验 (ELASA)及细胞免疫组织化学染色法检测脂质体 pSV2IFN β转染后 ,SHG4 4细胞IFN β的表达情况 ;应用四唑盐比色试验 (MTT)检测脂质体 pSV2IFN β转染后转染组与对照组SHG4 4细胞增殖的差异。 结果　IFN β基因脂质体pSV2IFN β转染SHG4 4胶质瘤细胞系后 4d和 6d ,对肿瘤细胞具有明显的增殖抑制作用 ,抑制率分别为 16 .7%和 32 .7%。ELASA及流式细胞仪蛋白检测均表明转染后胶质瘤细胞具有显著的IFN β表达 (P <0 .0 1) ,其中在转染后 72h和 96h ,IFN β表达量分别为 (35 .4± 2 .7)U/ml和 (4 0 .3± 3.2 )U/ml。免疫组织细胞化学实验也表明转染后的细胞有明显的IFN β的表达。 结论　IFN β基因脂质体 pSV2IFN β可转染SHG4 4胶质瘤细胞系 。Objective To investigate the transfection of human interferon-β gene liposome containing pSV2IFN-β to human glioma cell line SHG44 and the effects on their proliferation.Methods The interferon-β(IFN-β) expression in cell line SHG44 was observed by flow cytometry (FCM), enzyme linked immunosorbant assay(ELASA) and immunohistochemistry after transfection. 3-(4,5-dimethy1-2-thiazoly1)2, 5-diph- enyltetrazolium bromide(MTT) was used to measure the proliferation of SHG44 transfected with human interferon-β liposome containing pSV2IFN-β. Results The proliferation of SHG44 was obviously inhibited after transfection of human interferon-β liposome containing pSV2IFN-β. The inhibitory rates were 16.7% and 32.7% respectively after transfection 4 d and 6 d.The outcomes of FCM, ELASA and immunohistochemistry showed that the IFN-β expressions of SHG44 cells was significantly increased after transfections. The counts of IFN-β expression were (35.4±2.7) U/ml and (40.3±3.2) U/ml respectively after transfection 72 h and 96 h.Conclusion Human interferon-β gene liposome containing pSV2IFN-β can transfer into human glioma cell line SHG44 and inhibit the proliferation of cell line SHG44 significantly.

关 键 词：β-干扰素(IFN=β) 脂质体 转染 胶质瘤细胞 

分 类 号：R739.41[医药卫生—肿瘤]