人胰腺组织及胰腺癌细胞株蛋白质组的双向电泳分析  

Proteome study of human pancreatic cancer by two-dimensional gel electrophoresis

作　　者：李兴华[1] 徐云虹[2] 龚燕芳[1] 满晓华[1] 李兆申[1] 屠振兴[1] 

机构地区：[1]第二军医大学长海医院消化内科,上海200433 [2]吉林大学附属第二医院

出　　处：《胰腺病学》2004年第2期78-81,共4页Chinese JOurnal of Pancreatology

基　　金：国家自然科学基金资助项目( No.3 0 3 70 64 6)

摘　　要：目的　建立具有高分辨率和稳定性的胰腺癌蛋白质组研究的双向电泳方法。方法　取正常胰腺、胰腺癌手术标本和胰腺癌细胞株 ,分别制备组织、细胞匀浆 ,提取蛋白质组分 ,进行双向电泳。结果　加大蛋白酶抑制剂 ,应用两性电解质 ,可以得到理想的胰腺组织、细胞的蛋白质组分 ,并获得清晰、重复性良好的双向电泳图谱。结论　胰腺组织细胞蛋白质双向电泳中 ,样本的制备和双向电泳的条件是关键因素。Objective To establish two-dimensional gel electrophoresis with high resolution and reproducibility for proteomic study of pancreatic cancer.Methods Normal pancreas and surgically resected pancreatic cancer specimens and pancreatic cancer cell lines were homogenized with lysis solution. Proteome was detected by two-dimensional gel electrophoresis.Results Protein compositions of tissues and cells were well abstracted by increased protease inhibitors and pharmalytes. Two-dimensional gel electrophoretograms were clear and reproductive. Conclusions Sample preparation and electrophoresis condition were the key events for two-dimensional gel electrophoresis.

关 键 词：胰腺癌 癌细胞 蛋白质组 双向电泳分析 平衡液 

分 类 号：R735.9[医药卫生—肿瘤]