鱼类精液超低温冷冻保存的研究展望  

PROSPECT OF CRYOPRESERVATION OF FISH SPERMATOZOA

作　　者：于海涛[1] 张秀梅[1] 陈超[2] 

机构地区：[1]中国海洋大学生命科学与技术学部水产学院,山东青岛266003 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《海洋湖沼通报》2004年第2期66-72,共7页Transactions of Oceanology and Limnology

基　　金：国家自然科学基金 (30 0 70 5 93) ;山东省自然科学基金 (Y2 0 0 2D10 )资助

摘　　要：精液的超低温冷冻保存是从上世纪 5 0年代开始的 ,此项技术发展至今已取得很大成就。国外学者的工作主要集中在冷水性鲑鳟鱼类及某些海水鱼上 ,而国内以研究四大家鱼及其他淡水鱼类精液的超低温保存居多 ,只有少数学者研究如真鲷、黑鲷、花鲈、大弹涂鱼等海水鱼类精液的超低温保存。本文就开展鱼类精液冷冻保存研究的意义、鱼类精子超低温保存的基本生物学原理 ,及有关鱼类精液冷冻保存的研究现状等作一综合评述。In the past few decades, methods for cryopreservation of spermatozoa have been rapidly developed, and cryopreservation of the spermatozoa of salmonid fishes and some sea fishes have been well studied. But in China, while the spermatozoa of the four major Chinese carps and other fresh water fishes have been cryopreserved, only a few sea fishes, such ao red seabream, black seabream, sea bass and mudskipper, have been cryopreserved. In this paper, the mechanism of cryodamage is discussed. The basic biological theory of cryopreservation, the current status and the importance of cryopreserving fish spermatozoa are described.

关 键 词：鱼类 精子 超低温保存 抗冻剂 

分 类 号：S917[农业科学—水产科学] Q954.4[生物学—动物学]