离子交换色谱法测定枇杷果实和叶片中的有机酸  

Determination of the organic acids from the fruit and leaf of loquat by ion-exchange chromatography

作　　者：陈发兴[1] 刘星辉[2] 林华影[3] 陈立松[2] 

机构地区：[1]福建农林大学亚热带果树研究所,福建福州350002 [2]福建农林大学园艺学院,福建福州350002 [3]福州市疾病预防控制中心,福建福州350004

出　　处：《福建农林大学学报（自然科学版）》2004年第2期195-199,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金资助项目(B0310008).

摘　　要：用离子交换色谱法对枇杷有机酸进行有效分离与测定,EG40淋洗液在线发生器能降低背景电导,获得较好的重现性和平稳的基线.测定结果表明,枇杷果实和叶片中有机酸组分和含量有较大差别.果实中以苹果酸为主,其次为奎尼酸和酒石酸,而柠檬酸、草酸和顺乌头酸等含量较低.叶片中以奎尼酸为主,苹果酸、柠檬酸和草酸含量很低.Organic acids in loquat samples were adequately determined by ion-exchange chromatography. The performing gradient elution with EG40 eluent generator was used to obtain a flat baseline and better reproducibility. Results showed that the malic acid was the top organic acid in fruit; the seconds were quinic acid and tartartic acid. The others as citric, oxalic, cis-aconitic acid were little in fruit. In leaves, the main organic acid was quinic acid, while citric, malic and oxalic acid were little.

关 键 词：离子交换色谱法 枇杷 果实 叶片 有机酸 柠檬酸 苹果酸 

分 类 号：S667.301[农业科学—果树学] Q503[农业科学—园艺学]