巴曲酶对P-选择素表达的影响  

The effects of batroxobin on the expression of P-selectin

作　　者：姚治平[1] 周东[1] 胡湘蜀[1] 蔡振利 

机构地区：[1]四川大学华西医学院第一附属医院神经内科,成都610041 [2]大庆市第四人民医院神经内科

出　　处：《卒中与神经疾病》2004年第3期165-168,共4页Stroke and Nervous Diseases

摘　　要：目的　动态观察巴曲酶对大鼠脑缺血 /再灌注局灶血管表面P 选择素表达变化的影响。方法　以ABC法显示使用巴曲酶后局灶血管表面P 选择素的阳性表达情况。结果　使用巴曲酶可以使缺血 /再灌注脑组织血管表面第 2 4h和第 4d时P 选择素的表达明显降低。结论　巴曲酶可以降低血管表面P 选择素的表达 ,对缺血脑细胞有保护作用。Objective To dynamically observe the expression of P selectin on vessel walls in the ischemia/reperfusion rat model and the influence of batroxobin on it.Methods The P seletin expression on vessel walls and the influence of batroxobin in the focal ischemic cerebral tissues was measured at 1 hour, 3 hours, 6 hours, 12 hours, 24 hours, 2 days, 3 days, 4 days, 7 days, and 14 days after the reperfusion respectively by immunohistochemical method.Results P selectin expression on vessel walls got the first apparent upregulation one to three hours after the reperfusion and the second one 4 days after the reperfusion.Batroxobin may decrease the expression at the first ( P < 0.001 ) and fourth day ( P < 0.05 ).Conclusions There are two phases of upregulation of the expression of P selectin on vessel walls in the cerebral ischemia/reperfusion focus.As an adhesion molecule, P selectin plays an important role in the ischemia/reperfusion progress.Batroxobin may decrease the expression of P selection on vessel walls and show its protective effects on endothelial cells.

关 键 词：缺血/再灌注 P-选择素 巴曲酶 免疫组化 

分 类 号：R741[医药卫生—神经病学与精神病学]