β-环糊精催化磷酰化氨基酸水解反应的电喷雾电离质谱研究  

Study of β-Cyclodextrin-catalyzed Hydrolysis of N-Phosphoryl Amino Acid by Electrospray Ionization Mass Spectrometry

作　　者：曹书霞[1] 徐军[1] 张建臣[1] 陈晓岚[1] 赵玉芬[1] 

机构地区：[1]郑州大学化学系化学生物重点实验室,河南郑州450052

出　　处：《质谱学报》2004年第2期84-87,共4页Journal of Chinese Mass Spectrometry Society

摘　　要：利用电喷雾离子阱质谱对 β-环糊精和 N-二异丙氧磷酰化氨基酸的相互作用进行了探讨。结果发现 :β-环糊精能够催化 N-二异丙氧磷酰化氨基酸的水解反应 ,并对 β-环糊精对 N-二异丙氧磷酰化氨基酸的水解反应机制进行了推测。而且还对β-环糊精和 N -二异丙氧磷酰化氨基酸以及氨基酸形成的复合物进行了碰撞诱导裂解 ( CID)研究 。Aqueous solutions contained N diisopropyloxyphosphoryl amino acids (DIPP AA) and β cyclodextrin ( β CD) were analyzed by use of electrospray ion trap mass spectrometry (ESI MS) in conjunction with tandem mass spectrometry (MS/MS). The result showed that β CD could catalyze hydrolysis reaction of DIPP AA to free amino acid (AA). DIPP AA could form β CD/DIPP AA complex with β CD in the aqueous solution. The hydrolysis products were also observed to form the single charged 1∶1 ( β CD/AA) complexes. They not only had some similar cleavage patterns, but also had their similar characteristic fragment ions. The catalyzed mechanism of hydrolysis reaction was assumed by the result.

关 键 词：Β-环糊精 催化 磷酰化氨基酸 水解反应 电喷雾电离质谱 

分 类 号：Q517[生物学—生物化学]