抑制褪黑素生物合成对大鼠Tau蛋白磷酸化的影响  

EFFECT OF INHIBITING MELATONIN BIOSYNTHESIS ON TAU PHOSPHORYLATION IN RATS

作　　者：朱铃强[1] 王少辉[1] 凌智群[1] 王建枝[1] 

机构地区：[1]华中科技大学同济医学院病理生理学系,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2004年第2期189-192,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助 (30 70 5 6 0;3992 5 0 12;30 10 0 0 5 7;30 170 2 2 1)

摘　　要：目的　研究抑制褪黑素的生物合成对大鼠海马Tau蛋白磷酸化的影响。方法　侧脑室注射氟哌啶醇并腹腔注射加强 ,利用免疫组化检测大鼠海马区域Tau蛋白磷酸化情况 ;HPLC检测血清中褪黑素水平。结果　模型组大鼠海马Tau蛋白在Ser199/Ser2 0 2和Ser396 /Ser4 0 4位点均发生异常过度磷酸化 ,褪黑素治疗组较模型组的磷酸化程度轻。结论　褪黑素水平的降低可能与AD样Tau蛋白异常过度磷酸化相关 ,外源性补充褪黑素可以减轻Tau蛋白的异常过度磷酸化。? Objective To investigate the in vivo effect of inhibiting melatonin biosynthesis on tau phosphorylationinrats. Method Lateral ventricular and intraperitonealinjections were used for the administration of haloperidol, Western blots and immunohistochemistry forthe phosphorylation of tau, and HPLC for detectingthe level of melatonin in serum.Result Abnormal hyperphosphorylation of Tau was found in the hippocampusat Ser199/Ser202and Ser396/Ser404sites in the model group, while melatonin treatment attenuated this phosphorylation. Conclusion Decreased melatonin may be involved in Alzheimer-like tau hyperphosphorylation, and a supplement of melatonin attenuates this abnormality.

关 键 词：阿尔茨海默病 褪黑素 微管蛋白 异常过度磷酸化 

分 类 号：R36[医药卫生—病理学]