P27^(kip1)、EGFR在星形胶质瘤中的表达与其对增殖活性的影响  

EXPRESSION OF P27~ (kip1) AND EGFR AND THEIR EFFECTS ON THE PROLIFERATIVE ACTIVITY OF ASTROCYTOMA

作　　者：郭艳琳[1] 乔建新[2] 李士瑛[1] 张乃珍[2] 

机构地区：[1]山西医科大学病理教研室,太原030001 [2]山西省人民医院,太原030012

出　　处：《中国组织化学与细胞化学杂志》2004年第2期218-222,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的　检测P2 7kip1与EGFR在星形胶质瘤中的表达 ,探讨其对增殖活性的影响 ,为治疗提供一定的依据。方法　采用免疫组化S P法检测星形胶质瘤EGFR、P2 7kip1的表达 ,每一例肿瘤用ki 6 7LI表示其增殖活性 ,应用SAS8 0统计软件进行结果分析。结果　 1.P2 7kip1表达水平与星形胶质瘤病理分级负相关 (rs=- 0 4 87,P <0 0 0 0 1)。 2 .对EGFR、P2 7kip1及Ki 6 7LI联合分析显示 :P2 7kip1(+)EGFR (- )组的ki 6 7LI (5 0 % )明显低于P2 7kip1(+)EGFR(+)、P2 7kip1(- )EGFR (+)、P2 7kip1(- )EGFR (- )组 (10 4 %、 10 4 %、 10 8% ) (P <0 0 5 )。结论　 1.P2 7kip1在星形胶质瘤的发生、发展过程中可能起抑制作用。 2 .P2 7kip1、EGFR对星形胶质瘤增殖活性起相互拮抗的作用。? Objective To investigate the expression of P27 kip1 and EGFR and their effect on the proliferative activity of astrocytoma and to provide some basis for the therapy of astrocytoma. Method Using immuohistochemical S-P technique, we investigated the expression of EGFR and P27 kip1 in resected specimens of 59 patients with astrocytoma and defined the proliferative activity of each tumor by quantifying Ki-67 antigen. The results were analysed by SAS version 8.0. Result The P27 kip1 expression level showed a negative correlation with the pathological grade of astrocytoma (r s-0.487, P<0.0001). When EGFR, P27 kip1 and Ki-67 were analyzed together, the results showed that, when compared with the Ki-67 average labeling index of group P27 kip1(+) EGFR(-) (5.0%), those of group P27 kip1(+) EGFR(+), group P27 kip1(-) EGFR(+) and group P27 kip1(-) EGFR(-) (10.4%, 10.4% and 10.8% respectively) were significantly higher (P<0.05). Conclusion P27 kip1 demonstrated a negative effect on the occurrence and development of astrocytoma. P27 kip1 and EGFR demonstrated antagonistic effects the proliferative activity of astrocytoma.

关 键 词：P27^KIP1 表皮生长因子受体 KI-67 星形胶质瘤 

分 类 号：R739.41[医药卫生—肿瘤]